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@ARTICLE{Do:139786,
author = {Do, Quynh Hoa and Wittlich, Marc and Glück, Julian and
Möckel, Luis and Willbold, Dieter and König, Bernd and
Heise, Henrike},
title = {{F}ull-length {V}pu and human {CD}4(372–433) in
phospholipid bilayers as seen by magic angle spinning {NMR}},
journal = {Biological chemistry},
volume = {394},
number = {11},
issn = {1437-4315},
address = {Berlin [u.a.]},
publisher = {de Gruyter},
reportid = {FZJ-2013-05758},
pages = {1453 - 1463},
year = {2013},
abstract = {HIV-1 Vpu and CD4(372-433), a peptide comprising the
transmembrane and cytoplasmic domain of human CD4, were
recombinantly expressed in Escherichia coli, uniformly
labeled with 13C and 15N isotopes, and separately
reconstituted into phospholipid bilayers. Highly resolved
dipolar cross-polarization (CP)-based solid-state NMR
spectra of the two transmembrane proteins were recorded
under magic angle sample spinning. Partial assignment of 13C
resonances was achieved. Site-specific assignments were
obtained for 13 amino acid residues of CD4(372-433) and two
Vpu residues. Additional amino acid type-specific
assignments were achieved for 10 amino acid spin systems for
both CD4(372-433) and Vpu. Further, structural flexibility
was probed with different dipolar recoupling techniques, and
the correct insertion of the transmembrane domains into the
lipid bilayers was confirmed by proton spin diffusion
experiments.},
cin = {ICS-6},
ddc = {540},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {452 - Structural Biology (POF2-452)},
pid = {G:(DE-HGF)POF2-452},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000325717100009},
doi = {10.1515/hsz-2013-0194},
url = {https://juser.fz-juelich.de/record/139786},
}