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000139939 1001_ $$0P:(DE-HGF)0$$aMichalek, Matthias$$b0
000139939 245__ $$aStructure and function of a unique pore-forming protein from a pathogenic acanthamoeba
000139939 260__ $$bNature Publishing Group$$c2013
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000139939 520__ $$aHuman pathogens often produce soluble protein toxins that generate pores inside membranes, resulting in the death of target cells and tissue damage. In pathogenic amoebae, this has been exemplified with amoebapores of the enteric protozoan parasite Entamoeba histolytica. Here we characterize acanthaporin, to our knowledge the first pore-forming toxin to be described from acanthamoebae, which are free-living, bacteria-feeding, unicellular organisms that are opportunistic pathogens of increasing importance and cause severe and often fatal diseases. We isolated acanthaporin from extracts of virulent Acanthamoeba culbertsoni by tracking its pore-forming activity, molecularly cloned the gene of its precursor and recombinantly expressed the mature protein in bacteria. Acanthaporin was cytotoxic for human neuronal cells and exerted antimicrobial activity against a variety of bacterial strains by permeabilizing their membranes. The tertiary structures of acanthaporin's active monomeric form and inactive dimeric form, both solved by NMR spectroscopy, revealed a currently unknown protein fold and a pH-dependent trigger mechanism of activation.
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000139939 7001_ $$0P:(DE-HGF)0$$aSönnichsen, Frank D$$b1
000139939 7001_ $$0P:(DE-HGF)0$$aWechselberger, Rainer$$b2
000139939 7001_ $$0P:(DE-Juel1)145681$$aDingley, Andrew$$b3
000139939 7001_ $$0P:(DE-HGF)0$$aHung, Chien-Wen$$b4
000139939 7001_ $$0P:(DE-HGF)0$$aKopp, Annika$$b5
000139939 7001_ $$0P:(DE-HGF)0$$aWienk, Hans$$b6
000139939 7001_ $$0P:(DE-HGF)0$$aSimanski, Maren$$b7
000139939 7001_ $$0P:(DE-HGF)0$$aHerbst, Rosa$$b8
000139939 7001_ $$0P:(DE-HGF)0$$aLorenzen, Inken$$b9
000139939 7001_ $$0P:(DE-HGF)0$$aMarciano-Cabral, Francine$$b10
000139939 7001_ $$0P:(DE-HGF)0$$aGelhaus, Christoph$$b11
000139939 7001_ $$0P:(DE-HGF)0$$aGutsmann, Thomas$$b12
000139939 7001_ $$0P:(DE-HGF)0$$aTholey, Andreas$$b13
000139939 7001_ $$0P:(DE-HGF)0$$aGrötzinger, Joachim$$b14
000139939 7001_ $$0P:(DE-HGF)0$$aLeippe, Matthias$$b15$$eCorresponding author
000139939 773__ $$0PERI:(DE-600)2190276-8$$a10.1038/nchembio.1116$$gVol. 9, no. 1, p. 37 - 42$$n1$$p37 - 42$$tNature Chemical Biology$$v9$$x1552-4469$$y2012
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