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| Hauptseite > Publikationsdatenbank > The centrosomal adaptor TACC3 and the microtubule polymerase chTOG interact via defined C-terminal subdomains in an Aurora-A kinase independent manner > print |
| Hauptseite > Publikationsdatenbank > The centrosomal adaptor TACC3 and the microtubule polymerase chTOG interact via defined C-terminal subdomains in an Aurora-A kinase independent manner > print |
| 001 | 139998 | ||
| 005 | 20210129212737.0 | ||
| 024 | 7 | _ | |a 10.1074/jbc.M113.532333 |2 doi |
| 024 | 7 | _ | |a 1083-351X |2 ISSN |
| 024 | 7 | _ | |a 0021-9258 |2 ISSN |
| 024 | 7 | _ | |a WOS:000329370900007 |2 WOS |
| 037 | _ | _ | |a FZJ-2013-05965 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |a Thakur, H. C. |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a The centrosomal adaptor TACC3 and the microtubule polymerase chTOG interact via defined C-terminal subdomains in an Aurora-A kinase independent manner |
| 260 | _ | _ | |a Bethesda, Md. |c 2013 |b Soc. |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1385650879_8527 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 500 | _ | _ | |3 POF3_Assignment on 2016-02-29 |
| 520 | _ | _ | |a The cancer-associated, centrosomal adaptor protein TACC3 (Transforming Acidic Coiled-Coil 3) and its direct effector, the microtubule polymerase chTOG (colonic and hepatic tumor overexpressed gene), play a crucial function in centrosome-driven mitotic spindle assembly. It is unclear how TACC3 interacts with chTOG. Here, we show that the C-terminal TACC domain of TACC3 and a C-terminal fragment adjacent to the TOG domains of chTOG mediate the interaction between these two proteins. Interestingly, the TACC domain consists of two functionally distinct subdomains, CC1 (aa 414-530) and CC2 (aa 530-630). Whereas CC1 is responsible for the interaction with chTOG, CC2 performs an intradomain interaction with the central repeat region of TACC3, thereby masking the TACC domain prior to effector binding. Contrary to previous findings, our data clearly demonstrate that Aurora-A kinase does not regulate TACC3-chTOG complex formation, indicating that Aurora-A solely functions as a recruitment factor for the TACC3-chTOG complex to centrosomes and proximal mitotic spindles. We identified with CC1 and CC2 two functionally diverse modules within the TACC domain of TACC3 which mediate and modulate, respectively, TACC3 interaction with chTOG required for spindle assembly and microtubule dynamics during mitotic cell division. |
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| 700 | 1 | _ | |a Singh, M. |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Nagel-Steger, L. |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Kremer, J. |0 P:(DE-HGF)0 |b 3 |
| 700 | 1 | _ | |a Prumbaum, D. |0 P:(DE-HGF)0 |b 4 |
| 700 | 1 | _ | |a Kalawy Fansa, E. |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a Ezzahoini, H. |0 P:(DE-HGF)0 |b 6 |
| 700 | 1 | _ | |a Nouri, K. |0 P:(DE-HGF)0 |b 7 |
| 700 | 1 | _ | |a Gremer, L. |0 P:(DE-Juel1)145165 |b 8 |u fzj |
| 700 | 1 | _ | |a Abts, A. |0 P:(DE-HGF)0 |b 9 |
| 700 | 1 | _ | |a Schmitt, L. |0 P:(DE-HGF)0 |b 10 |
| 700 | 1 | _ | |a Raunser, S. |0 P:(DE-HGF)0 |b 11 |
| 700 | 1 | _ | |a Ahmadian, M. R. |0 P:(DE-HGF)0 |b 12 |
| 700 | 1 | _ | |a Piekorz, R. P. |0 P:(DE-HGF)0 |b 13 |e Corresponding author |
| 773 | _ | _ | |a 10.1074/jbc.M113.532333 |g p. M113.532333 |p - |0 PERI:(DE-600)1474604-9 |t The @journal of biological chemistry |v - |x 1083-351X |
| 856 | 4 | _ | |u http://www.jbc.org/content/early/2013/11/22/jbc.M113.532333.abstract |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/139998/files/FZJ-2013-05965.pdf |y Restricted |
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