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000014173 0247_ $$2DOI$$a10.1016/j.jphotobiol.2011.01.003
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000014173 084__ $$2WoS$$aBiochemistry & Molecular Biology
000014173 084__ $$2WoS$$aBiophysics
000014173 1001_ $$0P:(DE-Juel1)129358$$aMatsubara, S.$$b0$$uFZJ
000014173 245__ $$aPhotosystem II fluorescence lifetime imaging in avocado leaves: Contributions of the lutein-epoxide and violaxanthin cycles to fluorescence quenching
000014173 260__ $$aNew York, NY [u.a.]$$bElsevier$$c2011
000014173 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000014173 440_0 $$010861$$aJournal of Photochemistry and Photobiology B - Biology$$v104$$x1011-1344$$y1
000014173 500__ $$aThe research stay of S.M. at University of Illinois at Urbana-Champaign was supported by a Deutsche Akademische Austauschdienst (DAAD) travel grant (PPP-USA, D/07/10566). Y.-C.C. was supported by the Taiwan Merit Scholarships (TMS-094-1-A-036). G. was supported by the Department of Plant Biology at the University of Illinois at Urbana-Champaign. We thank Kelly Gillespie and Lisa Ainsworth (Department of Plant Biology, University of Illinois at Urbana-Champaign) for their help in freeze-drying the leaf disc samples. R.M.C. thanks the Research Board at UIUC for support. Although data were not included in this work, friendly and expert assistance by Mayandi Sivaguru (Microscopy and Imaging Facility, Institute for Genomic Biology, University of Illinois at Urbana-Champaign) for spinning disc confocal microscopy experiments is greatly acknowledged.
000014173 520__ $$aLifetime-resolved imaging measurements of chlorophyll a fluorescence were made on leaves of avocado plants to study whether rapidly reversible ΔpH-dependent (transthylakoid H(+) concentration gradient) thermal energy dissipation (qE) and slowly reversible ΔpH-independent fluorescence quenching (qI) are modulated by lutein-epoxide and violaxanthin cycles operating in parallel. Under normal conditions (without inhibitors), analysis of the chlorophyll a fluorescence lifetime data revealed two major lifetime pools (1.5 and 0.5 ns) for photosystem II during the ΔpH build-up under illumination. Formation of the 0.5-ns pool upon illumination was correlated with dark-retention of antheraxanthin and photo-converted lutein in leaves. Interconversion between the 1.5- and 0.5-ns lifetime pools took place during the slow part of the chlorophyll a fluorescence transient: first from 1.5 ns to 0.5 ns in the P-to-S phase, then back from 0.5 ns to 1.5 ns in the S-to-M phase. When linear electron transport and the resulting ΔpH build-up were inhibited by treatment with 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), the major fluorescence intensity was due to a 2.2-ns lifetime pool with a minor faster contribution of approximately 0.7 ns. In the presence of DCMU, neither the intensity nor the lifetimes of fluorescence were affected by antheraxanthin and photo-converted lutein. Thus, we conclude that both antheraxanthin and photo-converted lutein are able to enhance ΔpH-dependent qE processes that are associated with the 0.5-ns lifetime pool. However, unlike zeaxanthin, retention of antheraxanthin and photo-converted lutein may not by itself stabilize quenching or cause qI.
000014173 536__ $$0G:(DE-Juel1)FUEK407$$2G:(DE-HGF)$$aTerrestrische Umwelt$$cP24$$x0
000014173 588__ $$aDataset connected to Web of Science, Pubmed
000014173 65320 $$2Author$$aFluorescence lifetime imaging microscopy
000014173 65320 $$2Author$$aLutein
000014173 65320 $$2Author$$aLutein epoxide
000014173 65320 $$2Author$$aPolar plot
000014173 65320 $$2Author$$aThermal energy dissipation
000014173 65320 $$2Author$$aXanthophyll cycle
000014173 650_2 $$2MeSH$$aDiuron: pharmacology
000014173 650_2 $$2MeSH$$aHydrogen-Ion Concentration
000014173 650_2 $$2MeSH$$aLight
000014173 650_2 $$2MeSH$$aLutein: chemistry
000014173 650_2 $$2MeSH$$aMicroscopy, Fluorescence
000014173 650_2 $$2MeSH$$aPersea: enzymology
000014173 650_2 $$2MeSH$$aPhotosystem II Protein Complex: chemistry
000014173 650_2 $$2MeSH$$aPhotosystem II Protein Complex: metabolism
000014173 650_2 $$2MeSH$$aPlant Leaves: drug effects
000014173 650_2 $$2MeSH$$aPlant Leaves: enzymology
000014173 650_2 $$2MeSH$$aTime Factors
000014173 650_2 $$2MeSH$$aXanthophylls: chemistry
000014173 650_7 $$00$$2NLM Chemicals$$aPhotosystem II Protein Complex
000014173 650_7 $$00$$2NLM Chemicals$$aXanthophylls
000014173 650_7 $$0126-29-4$$2NLM Chemicals$$aviolaxanthin
000014173 650_7 $$0127-40-2$$2NLM Chemicals$$aLutein
000014173 650_7 $$0330-54-1$$2NLM Chemicals$$aDiuron
000014173 650_7 $$2WoSType$$aJ
000014173 7001_ $$0P:(DE-HGF)0$$aChen, Y.-C.$$b1
000014173 7001_ $$0P:(DE-Juel1)VDB90919$$aCaliandro, R.$$b2$$uFZJ
000014173 7001_ $$0P:(DE-HGF)0$$aGovindjee, G.$$b3
000014173 7001_ $$0P:(DE-Juel1)VDB90921$$aClegg, R.M.$$b4$$uFZJ
000014173 773__ $$0PERI:(DE-600)1482691-4$$a10.1016/j.jphotobiol.2011.01.003$$gVol. 104$$q104$$tJournal of photochemistry and photobiology / B$$v104$$x1011-1344$$y2011
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