Home > Publications database > Photosystem II fluorescence lifetime imaging in avocado leaves: Contributions of the lutein-epoxide and violaxanthin cycles to fluorescence quenching > print

001     14173
005     20200423202939.0
024 7 _ |2 pmid
|a pmid:21356597
024 7 _ |2 DOI
|a 10.1016/j.jphotobiol.2011.01.003
024 7 _ |2 WOS
|a WOS:000292066000027
037 _ _ |a PreJuSER-14173
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Biochemistry & Molecular Biology
084 _ _ |2 WoS
|a Biophysics
100 1 _ |0 P:(DE-Juel1)129358
|a Matsubara, S.
|b 0
|u FZJ
245 _ _ |a Photosystem II fluorescence lifetime imaging in avocado leaves: Contributions of the lutein-epoxide and violaxanthin cycles to fluorescence quenching
260 _ _ |a New York, NY [u.a.]
|b Elsevier
|c 2011
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |0 10861
|a Journal of Photochemistry and Photobiology B - Biology
|v 104
|x 1011-1344
|y 1
500 _ _ |a The research stay of S.M. at University of Illinois at Urbana-Champaign was supported by a Deutsche Akademische Austauschdienst (DAAD) travel grant (PPP-USA, D/07/10566). Y.-C.C. was supported by the Taiwan Merit Scholarships (TMS-094-1-A-036). G. was supported by the Department of Plant Biology at the University of Illinois at Urbana-Champaign. We thank Kelly Gillespie and Lisa Ainsworth (Department of Plant Biology, University of Illinois at Urbana-Champaign) for their help in freeze-drying the leaf disc samples. R.M.C. thanks the Research Board at UIUC for support. Although data were not included in this work, friendly and expert assistance by Mayandi Sivaguru (Microscopy and Imaging Facility, Institute for Genomic Biology, University of Illinois at Urbana-Champaign) for spinning disc confocal microscopy experiments is greatly acknowledged.
520 _ _ |a Lifetime-resolved imaging measurements of chlorophyll a fluorescence were made on leaves of avocado plants to study whether rapidly reversible ΔpH-dependent (transthylakoid H(+) concentration gradient) thermal energy dissipation (qE) and slowly reversible ΔpH-independent fluorescence quenching (qI) are modulated by lutein-epoxide and violaxanthin cycles operating in parallel. Under normal conditions (without inhibitors), analysis of the chlorophyll a fluorescence lifetime data revealed two major lifetime pools (1.5 and 0.5 ns) for photosystem II during the ΔpH build-up under illumination. Formation of the 0.5-ns pool upon illumination was correlated with dark-retention of antheraxanthin and photo-converted lutein in leaves. Interconversion between the 1.5- and 0.5-ns lifetime pools took place during the slow part of the chlorophyll a fluorescence transient: first from 1.5 ns to 0.5 ns in the P-to-S phase, then back from 0.5 ns to 1.5 ns in the S-to-M phase. When linear electron transport and the resulting ΔpH build-up were inhibited by treatment with 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), the major fluorescence intensity was due to a 2.2-ns lifetime pool with a minor faster contribution of approximately 0.7 ns. In the presence of DCMU, neither the intensity nor the lifetimes of fluorescence were affected by antheraxanthin and photo-converted lutein. Thus, we conclude that both antheraxanthin and photo-converted lutein are able to enhance ΔpH-dependent qE processes that are associated with the 0.5-ns lifetime pool. However, unlike zeaxanthin, retention of antheraxanthin and photo-converted lutein may not by itself stabilize quenching or cause qI.
536 _ _ |0 G:(DE-Juel1)FUEK407
|2 G:(DE-HGF)
|a Terrestrische Umwelt
|c P24
|x 0
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Diuron: pharmacology
650 _ 2 |2 MeSH
|a Hydrogen-Ion Concentration
650 _ 2 |2 MeSH
|a Light
650 _ 2 |2 MeSH
|a Lutein: chemistry
650 _ 2 |2 MeSH
|a Microscopy, Fluorescence
650 _ 2 |2 MeSH
|a Persea: enzymology
650 _ 2 |2 MeSH
|a Photosystem II Protein Complex: chemistry
650 _ 2 |2 MeSH
|a Photosystem II Protein Complex: metabolism
650 _ 2 |2 MeSH
|a Plant Leaves: drug effects
650 _ 2 |2 MeSH
|a Plant Leaves: enzymology
650 _ 2 |2 MeSH
|a Time Factors
650 _ 2 |2 MeSH
|a Xanthophylls: chemistry
650 _ 7 |0 0
|2 NLM Chemicals
|a Photosystem II Protein Complex
650 _ 7 |0 0
|2 NLM Chemicals
|a Xanthophylls
650 _ 7 |0 126-29-4
|2 NLM Chemicals
|a violaxanthin
650 _ 7 |0 127-40-2
|2 NLM Chemicals
|a Lutein
650 _ 7 |0 330-54-1
|2 NLM Chemicals
|a Diuron
650 _ 7 |2 WoSType
|a J
653 2 0 |2 Author
|a Fluorescence lifetime imaging microscopy
653 2 0 |2 Author
|a Lutein
653 2 0 |2 Author
|a Lutein epoxide
653 2 0 |2 Author
|a Polar plot
653 2 0 |2 Author
|a Thermal energy dissipation
653 2 0 |2 Author
|a Xanthophyll cycle
700 1 _ |0 P:(DE-HGF)0
|a Chen, Y.-C.
|b 1
700 1 _ |0 P:(DE-Juel1)VDB90919
|a Caliandro, R.
|b 2
|u FZJ
700 1 _ |0 P:(DE-HGF)0
|a Govindjee, G.
|b 3
700 1 _ |0 P:(DE-Juel1)VDB90921
|a Clegg, R.M.
|b 4
|u FZJ
773 _ _ |0 PERI:(DE-600)1482691-4
|a 10.1016/j.jphotobiol.2011.01.003
|g Vol. 104
|q 104
|t Journal of photochemistry and photobiology / B
|v 104
|x 1011-1344
|y 2011
856 4 _ |u https://juser.fz-juelich.de/record/14173/files/FZJ-14173.pdf
|z Published final document.
|y Restricted
909 C O |o oai:juser.fz-juelich.de:14173
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913 1 _ |0 G:(DE-Juel1)FUEK407
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|v Plant Science
|x 0
914 1 _ |y 2011
915 _ _ |0 StatID:(DE-HGF)0010
|a JCR/ISI refereed
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