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@INPROCEEDINGS{Rinklin:141739,
      author       = {Rinklin, Philipp and Afanasenkau, Dzmitry and Wiegand,
                      Simone and Wolfrum, Bernhard},
      title        = {{M}icrowire crossbar arrays for on-chip localized thermal
                      lesion of cell cultures},
      reportid     = {FZJ-2014-00105},
      year         = {2013},
      abstract     = {Driven by an advance in microfabrication technologies, the
                      development of miniaturized analytical platforms has become
                      a major interest in physical, chemical, and biological
                      research over the past two decades. On one hand, these
                      systems offer the possibility to massively decrease the
                      amount of resources and time necessary for current
                      point-of-care medical diagnostics. On the other hand, the
                      possibility to interact with biological systems in a highly
                      controlled and easily parallelizable manner offers many
                      promising opportunities for fundamental biological and
                      biophysical research. For example, when studying the healing
                      process of complex tissues after lesion, the use of
                      simplified in vitro models can help to elucidate basic
                      mechanisms. In this context, a means to create these lesions
                      with high spatial control and resolution is of great
                      importance. While the use of lasers coupled to microscopes
                      is capable of delivering the necessary control and
                      resolution, the requirement of external optics renders an
                      application to on-chip devices difficult. Here, we
                      demonstrate the use of microwire crossbar chips for the
                      generation of localized thermally induced lesions in on-chip
                      tissue models. Our chips consist of two orthogonal layers of
                      parallel microwires, insulated from the culture medium by a
                      polyimide layer. Cardiomyocyte-like HL-1 cells are cultured
                      on the chip as an in vitro tissue model. Passing an
                      electrical current through a given set of microwires leads
                      to thermal heating of the active wires, which consequently
                      imposes a localized stress on the cells cultured at the
                      chip’s surface. We demonstrate that using this method,
                      complex lesion patterns with a resolution in the lower
                      micrometer regime can be created. The success of the lesion
                      as well as the effects on the surrounding cells are
                      evaluated using Calcein/EtHD staining methods. We further
                      analyze the distinct Ca2+ propagation inside the cell layer
                      revealing partially decoupled network activity depending on
                      the applied lesion patterns. In conclusion, we believe that
                      our method can be used as a versatile tool to study tissue
                      lesions in simplified model systems. As a chip-based method,
                      it also allows for low-cost production, as well as
                      straight-forward inclusion in microsystems, which
                      facilitates high-throughput and the generation of
                      statistically relevant data from biological systems prone to
                      high noise levels.},
      month         = {Nov},
      date          = {2013-11-18},
      organization  = {NanoBioTech Montreux, Montreux
                       (Switzerland), 18 Nov 2013 - 20 Nov
                       2013},
      subtyp        = {After Call},
      cin          = {PGI-8 / ICS-8 / JARA-FIT / ICS-3},
      cid          = {I:(DE-Juel1)PGI-8-20110106 / I:(DE-Juel1)ICS-8-20110106 /
                      I:(DE-Juel1)VDB881 / I:(DE-Juel1)ICS-3-20110106},
      pnm          = {453 - Physics of the Cell (POF2-453) / 423 - Sensorics and
                      bioinspired systems (POF2-423) / 452 - Structural Biology
                      (POF2-452)},
      pid          = {G:(DE-HGF)POF2-453 / G:(DE-HGF)POF2-423 /
                      G:(DE-HGF)POF2-452},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/141739},
}