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@ARTICLE{Accardo:14184,
author = {Accardo, A. and Morisco, A. and Gianolio, E. and Tesauro,
D. and Mangiapia, G. and Radulescu, A. and Brandt, A. and
Morelli, G.},
title = {{N}anoparticles containing octeotride peptides and
gadolinium complexes for {MRI} applications},
journal = {Journal of peptide science},
volume = {17},
issn = {1075-2617},
address = {New York, NY [u.a.]},
publisher = {Wiley},
reportid = {PreJuSER-14184},
pages = {154 - 162},
year = {2011},
note = {Record converted from VDB: 12.11.2012},
abstract = {New mixed nanoparticles were obtained by self-aggregation
of two amphiplic monomers. The first monomer (C18)(2) L5-Oct
contains two C18 hydrophobic moieties bound to the
N-terminus of the cyclic peptide octreotide, and spaced from
the bioactive peptide by five units of dioxoethylene
linkers. The second monomer, (C18)(2) DTPAGlu, (C18)(2) DTPA
or (C18)(2) DOTA, and the corresponding Gd(III) complexes,
contains two C18 hydrophobic moieties bound through a lysine
residue to different polyamino-polycarboxy ligands: DTPAGlu,
DTPA or DOTA. Mixed aggregates have been obtained and
structurally characterized by small angle neutron scattering
(SANS) techniques and for their relaxometric behavior.
According to a decrease of negative charges in the
surfactant head-group, a total or a partial
micelle-to-vesicle transition is observed by passing from
(C18)(2) DTPAGlu to (C18)(2) DOTA. The thicknesses of the
bilayers are substantially constant, around 50 Å, in the
analyzed systems. Moreover, the mixed aggregates, in which a
small amount of amphiphilic octreotide monomer (C18)(2)
L5-Oct $(10\%$ mol/mol) was inserted, do not differ
significantly from the respective self-assembled systems.
Fluorescence emission of tryptophan residue at 340 nm
indicates low mobility of water molecules at the peptide
surface. The proton relaxivity of mixed aggregates based on
(C18)(2) DTPAGlu(Gd), (C18)(2) DTPA(Gd) and (C18)(2)
DOTA(Gd) resulted to be 17.6, 15.2 and 10.0 mM(-1) s(-1) (at
20 MHz and 298K), respectively. The decrease in the
relaxivity values can be ascribed to the increase in τ(M)
(81, 205 and 750 ns). The presence of amphiphilic octreotide
monomer exposed on mixed aggregate surface gives the entire
nanoparticles a potential binding selectivity toward
somatostatin sstr2 receptor subtype, and these systems could
act as MRI target-specific contrast agent.},
keywords = {Contrast Media: chemistry / Gadolinium: chemistry /
Magnetic Resonance Imaging: methods / Nanoparticles:
chemistry / Octreotide: chemistry / Contrast Media (NLM
Chemicals) / Gadolinium (NLM Chemicals) / Octreotide (NLM
Chemicals) / J (WoSType)},
cin = {PGI-4 / ICS-1 / Jülich Centre for Neutron Science JCNS
(JCNS) ; JCNS},
ddc = {570},
cid = {I:(DE-Juel1)PGI-4-20110106 / I:(DE-Juel1)ICS-1-20110106 /
I:(DE-Juel1)JCNS-20121112},
pnm = {BioSoft: Makromolekulare Systeme und biologische
Informationsverarbeitung / Großgeräte für die Forschung
mit Photonen, Neutronen und Ionen (PNI)},
pid = {G:(DE-Juel1)FUEK505 / G:(DE-Juel1)FUEK415},
experiment = {EXP:(DE-MLZ)KWS2-20140101},
shelfmark = {Biochemistry $\&$ Molecular Biology / Chemistry,
Analytical},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:21234988},
UT = {WOS:000287164000013},
doi = {10.1002/psc.1308},
url = {https://juser.fz-juelich.de/record/14184},
}