TY  - JOUR
AU  - Pacheco, V.
AU  - Ma, P.
AU  - Thielmann, Y.
AU  - Hartmann, R.
AU  - Weiergräber, O.H.
AU  - Mohrlüder, J.
AU  - Willbold, D.
TI  - Assessment of GABARAP self-association by its diffusion properties
JO  - Journal of biomolecular NMR
VL  - 48
SN  - 0925-2738
CY  - Dordrecht [u.a.]
PB  - Springer Science + Business Media B.V
M1  - PreJuSER-14240
SP  - 49 - 58
PY  - 2010
N1  - O. H. Weiergraber is grateful to Georg Buldt for continuous generous support. V. Pacheco appreciates support by Heike Schneider. This work was supported by a Deutsche Forschungsgemeinschaft (DFG) grant to D. W. (Wi1472/5) and a PhD scholarship from the Deutscher Akademischer Austauschdienst (DAAD) to P. M.
AB  - Gamma-aminobutyric acid type A receptor-associated protein (GABARAP) belongs to a family of small ubiquitin-like adaptor proteins implicated in intracellular vesicle trafficking and autophagy. We have used diffusion-ordered nuclear magnetic resonance spectroscopy to study the temperature and concentration dependence of the diffusion properties of GABARAP. Our data suggest the presence of distinct conformational states and provide support for self-association of GABARAP molecules. Assuming a monomer-dimer equilibrium, a temperature-dependent dissociation constant could be derived. Based on a temperature series of (1)H(15)N heteronuclear single quantum coherence nuclear magnetic resonance spectra, we propose residues potentially involved in GABARAP self-interaction. The possible biological significance of these observations is discussed with respect to alternative scenarios of oligomerization.
KW  - Adaptor Proteins, Signal Transducing: chemistry
KW  - Adaptor Proteins, Signal Transducing: metabolism
KW  - Diffusion
KW  - Humans
KW  - Microtubule-Associated Proteins: chemistry
KW  - Microtubule-Associated Proteins: metabolism
KW  - Models, Molecular
KW  - Nuclear Magnetic Resonance, Biomolecular: methods
KW  - Protein Multimerization
KW  - Temperature
KW  - Adaptor Proteins, Signal Transducing (NLM Chemicals)
KW  - GABARAP protein, human (NLM Chemicals)
KW  - Microtubule-Associated Proteins (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:20665069
UR  - <Go to ISI:>//WOS:000282102300005
DO  - DOI:10.1007/s10858-010-9437-5
UR  - https://juser.fz-juelich.de/record/14240
ER  -