% IMPORTANT: The following is UTF-8 encoded. This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.
@ARTICLE{Pacheco:14240,
author = {Pacheco, V. and Ma, P. and Thielmann, Y. and Hartmann, R.
and Weiergräber, O.H. and Mohrlüder, J. and Willbold, D.},
title = {{A}ssessment of {GABARAP} self-association by its diffusion
properties},
journal = {Journal of biomolecular NMR},
volume = {48},
issn = {0925-2738},
address = {Dordrecht [u.a.]},
publisher = {Springer Science + Business Media B.V},
reportid = {PreJuSER-14240},
pages = {49 - 58},
year = {2010},
note = {O. H. Weiergraber is grateful to Georg Buldt for continuous
generous support. V. Pacheco appreciates support by Heike
Schneider. This work was supported by a Deutsche
Forschungsgemeinschaft (DFG) grant to D. W. (Wi1472/5) and a
PhD scholarship from the Deutscher Akademischer
Austauschdienst (DAAD) to P. M.},
abstract = {Gamma-aminobutyric acid type A receptor-associated protein
(GABARAP) belongs to a family of small ubiquitin-like
adaptor proteins implicated in intracellular vesicle
trafficking and autophagy. We have used diffusion-ordered
nuclear magnetic resonance spectroscopy to study the
temperature and concentration dependence of the diffusion
properties of GABARAP. Our data suggest the presence of
distinct conformational states and provide support for
self-association of GABARAP molecules. Assuming a
monomer-dimer equilibrium, a temperature-dependent
dissociation constant could be derived. Based on a
temperature series of (1)H(15)N heteronuclear single quantum
coherence nuclear magnetic resonance spectra, we propose
residues potentially involved in GABARAP self-interaction.
The possible biological significance of these observations
is discussed with respect to alternative scenarios of
oligomerization.},
keywords = {Adaptor Proteins, Signal Transducing: chemistry / Adaptor
Proteins, Signal Transducing: metabolism / Diffusion /
Humans / Microtubule-Associated Proteins: chemistry /
Microtubule-Associated Proteins: metabolism / Models,
Molecular / Nuclear Magnetic Resonance, Biomolecular:
methods / Protein Multimerization / Temperature / Adaptor
Proteins, Signal Transducing (NLM Chemicals) / GABARAP
protein, human (NLM Chemicals) / Microtubule-Associated
Proteins (NLM Chemicals) / J (WoSType)},
cin = {ISB-3 / JARA-HPC / ICS-5},
ddc = {570},
cid = {I:(DE-Juel1)VDB942 / $I:(DE-82)080012_20140620$ /
I:(DE-Juel1)ICS-5-20110106},
pnm = {Funktion und Dysfunktion des Nervensystems / BioSoft:
Makromolekulare Systeme und biologische
Informationsverarbeitung},
pid = {G:(DE-Juel1)FUEK409 / G:(DE-Juel1)FUEK505},
shelfmark = {Biochemistry $\&$ Molecular Biology / Spectroscopy},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:20665069},
UT = {WOS:000282102300005},
doi = {10.1007/s10858-010-9437-5},
url = {https://juser.fz-juelich.de/record/14240},
}