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000014241 084__ $$2WoS$$aBiology
000014241 1001_ $$0P:(DE-HGF)0$$aPanza, G.$$b0
000014241 245__ $$aMolecular interactions between prions as seeds and recombinant prion proteins as substrates resemble the biological interspecies barrier in vitro
000014241 260__ $$aLawrence, Kan.$$bPLoS$$c2010
000014241 300__ $$ae14283
000014241 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000014241 440_0 $$018181$$aPLOS One$$v5$$x1932-6203
000014241 500__ $$aThis authors were funded by the European Union: (Network of Excellence "NeuroPrion", FOOD-CT-2004-506579, http://www.neuroprion.org/en/index. html); "Presidentenfond der Helmholtzgemeinschaft" (HGF, "Virtual Institute of Structural Biology"), http://www.vibs-rhine-ruhr.org/; and Food Standards Agency UK (M03R0005/004) http://www.food.gov.uk/. Lars Luers was a fellow of the Graduiertenkolleg 1033. Jan Stohr was supported by a postdoctoral fellowship of the Deutsche Forschungsgemeinschaft (DFG). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
000014241 520__ $$aPrion diseases like Creutzfeldt-Jakob disease in humans, Scrapie in sheep or bovine spongiform encephalopathy are fatal neurodegenerative diseases, which can be of sporadic, genetic, or infectious origin. Prion diseases are transmissible between different species, however, with a variable species barrier. The key event of prion amplification is the conversion of the cellular isoform of the prion protein (PrP(C)) into the pathogenic isoform (PrP(Sc)). We developed a sodiumdodecylsulfate-based PrP conversion system that induces amyloid fibril formation from soluble α-helical structured recombinant PrP (recPrP). This approach was extended applying pre-purified PrP(Sc) as seeds which accelerate fibrillization of recPrP. In the present study we investigated the interspecies coherence of prion disease. Therefore we used PrP(Sc) from different species like Syrian hamster, cattle, mouse and sheep and seeded fibrillization of recPrP from the same or other species to mimic in vitro the natural species barrier. We could show that the in vitro system of seeded fibrillization is in accordance with what is known from the naturally occurring species barriers.
000014241 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000014241 536__ $$0G:(DE-Juel1)FUEK505$$2G:(DE-HGF)$$aBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$cP45$$x1
000014241 588__ $$aDataset connected to Web of Science, Pubmed
000014241 650_2 $$2MeSH$$aAmyloid: chemistry
000014241 650_2 $$2MeSH$$aAnimals
000014241 650_2 $$2MeSH$$aBrain: metabolism
000014241 650_2 $$2MeSH$$aCattle
000014241 650_2 $$2MeSH$$aCircular Dichroism
000014241 650_2 $$2MeSH$$aCricetinae
000014241 650_2 $$2MeSH$$aKinetics
000014241 650_2 $$2MeSH$$aMesocricetus
000014241 650_2 $$2MeSH$$aMice
000014241 650_2 $$2MeSH$$aNeurodegenerative Diseases: metabolism
000014241 650_2 $$2MeSH$$aPrion Diseases: genetics
000014241 650_2 $$2MeSH$$aPrion Diseases: metabolism
000014241 650_2 $$2MeSH$$aPrion Diseases: transmission
000014241 650_2 $$2MeSH$$aPrions: chemistry
000014241 650_2 $$2MeSH$$aProtein Structure, Secondary
000014241 650_2 $$2MeSH$$aRecombinant Proteins: chemistry
000014241 650_2 $$2MeSH$$aSheep
000014241 650_2 $$2MeSH$$aSodium Dodecyl Sulfate: chemistry
000014241 650_2 $$2MeSH$$aSpecies Specificity
000014241 650_2 $$2MeSH$$aUltracentrifugation
000014241 650_7 $$00$$2NLM Chemicals$$aAmyloid
000014241 650_7 $$00$$2NLM Chemicals$$aPrions
000014241 650_7 $$00$$2NLM Chemicals$$aRecombinant Proteins
000014241 650_7 $$0151-21-3$$2NLM Chemicals$$aSodium Dodecyl Sulfate
000014241 650_7 $$2WoSType$$aJ
000014241 7001_ $$0P:(DE-Juel1)VDB97355$$aLuers, L.$$b1$$uFZJ
000014241 7001_ $$0P:(DE-HGF)0$$aStöhr, J.$$b2
000014241 7001_ $$0P:(DE-HGF)0$$aNagel-Steger, L.$$b3
000014241 7001_ $$0P:(DE-HGF)0$$aWeiß, J.$$b4
000014241 7001_ $$0P:(DE-HGF)0$$aRiesner, D.$$b5
000014241 7001_ $$0P:(DE-Juel1)132029$$aWillbold, D.$$b6$$uFZJ
000014241 7001_ $$0P:(DE-Juel1)VDB65870$$aBirkmann, E.$$b7$$uFZJ
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