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000014335 0247_ $$2DOI$$a10.1016/j.ibmb.2010.05.004
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000014335 041__ $$aeng
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000014335 084__ $$2WoS$$aBiochemistry & Molecular Biology
000014335 084__ $$2WoS$$aEntomology
000014335 1001_ $$0P:(DE-Juel1)VDB93301$$aFuss, N.$$b0$$uFZJ
000014335 245__ $$aBiochemical properties of heterologously expressed and native adenylyl cyclases from the honeybee brain (Apis mellifera L.)
000014335 260__ $$aAmsterdam$$bElsevier$$c2010
000014335 300__ $$a573 - 580
000014335 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000014335 440_0 $$022582$$aInsect Biochemistry and Molecular Biology$$v40$$x0965-1748$$y8
000014335 500__ $$aWe gratefully acknowledge the technical assistance of S. Balfanz (Julich). For help with Western blots containing native honeybee proteins, we thank B. Troppmann (University Potsdam). This study was supported by grants BA 1541/6-1/2 and Er 79/6-2 from the Deutsche Forschungsgemeinschaft.
000014335 520__ $$aCyclic AMP is an important intracellular signaling molecule participating e.g. in sensory signal transduction, cardiac myocyte regulation, learning and memory. The formation of cAMP is catalyzed by adenylyl cyclases. A variety of factors can modulate the properties of these enzymes and lead to dynamic changes of the intracellular cAMP concentration. Here we determined the tissue distribution of a recently cloned adenylyl cyclase (AmAC3) in honeybee brain. The protein is present in all neuropils. Intensive immunoreactivity was found in parts of the proto- and deutocerebrum and in the suboesophageal ganglion. Biochemical and pharmacological properties of AmAC3 and of native adenylyl cyclases in subregions of the honeybee brain were examined. Values for half-maximal activation with NKH477 were in the low micromolar range with 10.2 μM for AmAC3 and 3.6-8.1 μM for native enzymes. Biosynthesis of cAMP was specifically blocked by P-site inhibitors. Adenylyl cyclases in antennal lobes and AmAC3 share the inhibitory profile with 2',5'dd3'ATP>3'AMP>2'deoxyadenosine. In addition to P-site inhibitors AmAC3 activity was impaired by Ca(2+)/calmodulin. The results suggest that AmAC3 is a likely candidate to fulfill an integrative role in sensory, motor and higher-order information processing in the honeybee brain.
000014335 536__ $$0G:(DE-Juel1)FUEK505$$2G:(DE-HGF)$$aBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$cP45$$x0
000014335 588__ $$aDataset connected to Web of Science, Pubmed
000014335 65320 $$2Author$$aCellular signaling
000014335 65320 $$2Author$$aLearning and memory
000014335 65320 $$2Author$$aSecond messenger
000014335 65320 $$2Author$$aCa2+/CaM
000014335 65320 $$2Author$$aP-site inhibitor
000014335 65320 $$2Author$$aForskolin
000014335 650_2 $$2MeSH$$aAdenylate Cyclase: chemistry
000014335 650_2 $$2MeSH$$aAdenylate Cyclase: genetics
000014335 650_2 $$2MeSH$$aAdenylate Cyclase: metabolism
000014335 650_2 $$2MeSH$$aAnimals
000014335 650_2 $$2MeSH$$aBees: enzymology
000014335 650_2 $$2MeSH$$aBees: genetics
000014335 650_2 $$2MeSH$$aBrain: enzymology
000014335 650_2 $$2MeSH$$aCell Line
000014335 650_2 $$2MeSH$$aEnzyme Activation
000014335 650_2 $$2MeSH$$aGene Expression
000014335 650_2 $$2MeSH$$aInsect Proteins: chemistry
000014335 650_2 $$2MeSH$$aInsect Proteins: genetics
000014335 650_2 $$2MeSH$$aInsect Proteins: metabolism
000014335 650_2 $$2MeSH$$aNeuropil: enzymology
000014335 650_2 $$2MeSH$$aProtein Transport
000014335 650_7 $$00$$2NLM Chemicals$$aInsect Proteins
000014335 650_7 $$0EC 4.6.1.1$$2NLM Chemicals$$aAdenylate Cyclase
000014335 650_7 $$2WoSType$$aJ
000014335 7001_ $$0P:(DE-HGF)0$$aMujagic, S.$$b1
000014335 7001_ $$0P:(DE-Juel1)VDB15958$$aWachten, S.$$b2$$uFZJ
000014335 7001_ $$0P:(DE-HGF)0$$aErber, J.$$b3
000014335 7001_ $$0P:(DE-Juel1)131911$$aBaumann, A.$$b4$$uFZJ
000014335 773__ $$0PERI:(DE-600)1483248-3$$a10.1016/j.ibmb.2010.05.004$$gVol. 40, p. 573 - 580$$p573 - 580$$q40<573 - 580$$tInsect biochemistry and molecular biology$$v40$$x0965-1748$$y2010
000014335 8567_ $$uhttp://dx.doi.org/10.1016/j.ibmb.2010.05.004
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000014335 9141_ $$y2010
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