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000014490 0247_ $$2pmid$$apmid:20678470
000014490 0247_ $$2DOI$$a10.1016/j.bbrc.2010.07.110
000014490 0247_ $$2WOS$$aWOS:000281840200017
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000014490 037__ $$aPreJuSER-14490
000014490 041__ $$aeng
000014490 082__ $$a570
000014490 084__ $$2WoS$$aBiochemistry & Molecular Biology
000014490 084__ $$2WoS$$aBiophysics
000014490 1001_ $$0P:(DE-Juel1)VDB26956$$aKüpper, K.$$b0$$uFZJ
000014490 245__ $$aTyrosine phosphorylation of vinculin at position 1065 modifies focal adhesion dynamics and cell tractions
000014490 260__ $$aOrlando, Fla.$$bAcademic Press$$c2010
000014490 300__ $$a560 - 564
000014490 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000014490 3367_ $$2BibTeX$$aARTICLE
000014490 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000014490 3367_ $$2DRIVER$$aarticle
000014490 440_0 $$0787$$aBiochemical and Biophysical Research Communications$$v399$$x0006-291X$$y4
000014490 500__ $$aWe thank Dr. E.D. Adamson for providing the MEF cells and Dr. B. Fabry for stimulating discussions. We are also indebted to the entire team of IBN 4 for their interlectual input. This work was supported by grants from Bayerisch-Franzosisches Hochschulzentrum, Deutscher Akademischer Austausch Dienst, Bavaria California Technology Center, Deutsche Forschungsgemeinschaft (G0598/13-1), and Bundesministerium fur Bildung und Forschung (Program 0315501).
000014490 520__ $$aFocal adhesions (FAs) connect the cellular actin cytoskeleton via integrin with the extracellular matrix. They comprise of many structural and signaling proteins which are highly dynamic, well regulated, and responsible for the sensing of physical properties from the environment. Vinculin is a protein that incorporates all these functions. Here, we investigated the phosphorylation of Y1065 in the activation/regulation of vinculin. We used different vinculin mutants mimicking either a permanently activated or inhibited phosphorylation site at position 1065. Using these mutants, we determined their influence on the exchange dynamics and cell forces using fluorescence recovery after photobleaching and traction microscopy. The results indicate that phosphorylation at Y1065 significantly increases the amount of freely exchanging vinculin within FAs whereas inhibition of this phosphorylation site leads to an uncontrolled exchange of vinculin and reduced adhesive cell forces. In conclusion, we show that phosphorylation on position Y1065 is essential for accurate incorporation of vinculin into FAs and mechanical behavior of cells.
000014490 536__ $$0G:(DE-Juel1)FUEK505$$2G:(DE-HGF)$$aBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$cP45$$x0
000014490 588__ $$aDataset connected to Web of Science, Pubmed
000014490 65320 $$2Author$$aVinculin
000014490 65320 $$2Author$$aFocal adhesion dynamics
000014490 65320 $$2Author$$aFluorescence recovery after photobleaching
000014490 65320 $$2Author$$aCell tractions
000014490 65320 $$2Author$$aTyrosine phosphorylation
000014490 650_2 $$2MeSH$$aAnimals
000014490 650_2 $$2MeSH$$aFocal Adhesions: genetics
000014490 650_2 $$2MeSH$$aFocal Adhesions: metabolism
000014490 650_2 $$2MeSH$$aMice
000014490 650_2 $$2MeSH$$aMice, Knockout
000014490 650_2 $$2MeSH$$aMutation
000014490 650_2 $$2MeSH$$aPhosphorylation
000014490 650_2 $$2MeSH$$aTyrosine: genetics
000014490 650_2 $$2MeSH$$aTyrosine: metabolism
000014490 650_2 $$2MeSH$$aVinculin: genetics
000014490 650_2 $$2MeSH$$aVinculin: metabolism
000014490 650_7 $$0125361-02-6$$2NLM Chemicals$$aVinculin
000014490 650_7 $$055520-40-6$$2NLM Chemicals$$aTyrosine
000014490 650_7 $$2WoSType$$aJ
000014490 7001_ $$0P:(DE-Juel1)VDB891$$aLang, N.$$b1$$uFZJ
000014490 7001_ $$0P:(DE-Juel1)VDB71075$$aMöhl, C.$$b2$$uFZJ
000014490 7001_ $$0P:(DE-Juel1)VDB8902$$aKirchgeßner, N.$$b3$$uFZJ
000014490 7001_ $$0P:(DE-Juel1)161241$$aBorn, S.$$b4$$uFZJ
000014490 7001_ $$0P:(DE-Juel1)VDB86495$$aGoldmann, W.H.$$b5$$uFZJ
000014490 7001_ $$0P:(DE-Juel1)VDB27696$$aHoffmann, B.$$b6$$uFZJ
000014490 7001_ $$0P:(DE-Juel1)128833$$aMerkel, R.$$b7$$uFZJ
000014490 773__ $$0PERI:(DE-600)1461396-7$$a10.1016/j.bbrc.2010.07.110$$gVol. 399, p. 560 - 564$$p560 - 564$$q399<560 - 564$$tBiochemical and biophysical research communications$$v399$$x0006-291X$$y2010
000014490 8567_ $$uhttp://dx.doi.org/10.1016/j.bbrc.2010.07.110
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000014490 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000014490 9141_ $$y2010
000014490 9131_ $$0G:(DE-Juel1)FUEK505$$aDE-HGF$$bSchlüsseltechnologien$$kP45$$lBiologische Informationsverarbeitung$$vBioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung$$x0
000014490 9132_ $$0G:(DE-HGF)POF3-552$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lBioSoft  Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vEngineering Cell Function$$x0
000014490 9201_ $$0I:(DE-Juel1)VDB802$$d31.12.2010$$gIBN$$kIBN-4$$lBiomechanik$$x0
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