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000001483 041__ $$aeng
000001483 082__ $$a610
000001483 084__ $$2WoS$$aNeurosciences
000001483 1001_ $$0P:(DE-Juel1)VDB79698$$aKnop, G.C.$$b0$$uFZJ
000001483 245__ $$aLight responses in the mouse retina are prolonged upon targeted deletion of the HCN1 channel gene
000001483 260__ $$aOxford [u.a.]$$bBlackwell$$c2008
000001483 300__ $$a2221 - 2230
000001483 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000001483 440_0 $$01951$$aEuropean Journal of Neuroscience$$v28$$x0953-816X
000001483 500__ $$aWe thank Dr Eric Kandel ( Columbia University, USA) for providing the HCN1 knock-out line, Christoph Aretzweiler for technical assistance in immunohistochemistry and genotyping of HCN1 knock-out animals, Mechthilde Bruns for help in cell culture, and Dr Wolfgang Bonigk for the cDNA of murine HCN1. M. W. Seeliger was supported by the Deutsche Forschungsgemeinschaft ( DFG Se837/4-1 and Se837/5-1).
000001483 520__ $$aHyperpolarization-activated and cyclic nucleotide-gated (HCN) channels contribute to pacemaker activity, and co-determine the integrative behaviour of neurons and shape their response to synaptic stimulation. Four channel isoforms, HCN1-4, have been described in mammals. Recent studies showed particularly strong expression of HCN1 channels in rods and cones of the rat retina, suggesting that HCN1 channels are involved in the shaping of light responses in both types of photoreceptors. Therefore, the loss of HCN1 channels should lead to pronounced changes in light-induced electrical responses under both scotopic and photopic conditions. This was tested using a mouse transgenic approach. We used immunohistochemistry and patch-clamp recording to study the distribution of HCN1 channels in the mouse retina. HCN1 channels were strongly expressed in rod and cone photoreceptors, as well as in some bipolar, amacrine and ganglion cell types. In electroretinograms (ERGs) from animals in which the HCN1 channel gene had been knocked out, the b-wave amplitudes were unaltered (scotopic conditions) or somewhat reduced (photopic conditions), whereas the duration of both scotopic and photopic ERG responses was strikingly prolonged. Our data suggest that in visual information processing, shortening and shaping of light responses by activation of HCN1 at the level of the photoreceptors is an important step in both scotopic and photopic pathways.
000001483 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000001483 588__ $$aDataset connected to Web of Science, Pubmed
000001483 65320 $$2Author$$aelectrophysiology
000001483 65320 $$2Author$$aelectroretinography
000001483 65320 $$2Author$$aERG
000001483 65320 $$2Author$$aHCN channels
000001483 65320 $$2Author$$aI-h
000001483 65320 $$2Author$$aretina
000001483 650_2 $$2MeSH$$aAmacrine Cells: metabolism
000001483 650_2 $$2MeSH$$aAmacrine Cells: radiation effects
000001483 650_2 $$2MeSH$$aAnimals
000001483 650_2 $$2MeSH$$aCyclic Nucleotide-Gated Cation Channels: genetics
000001483 650_2 $$2MeSH$$aElectroretinography
000001483 650_2 $$2MeSH$$aImmunohistochemistry
000001483 650_2 $$2MeSH$$aMembrane Potentials: genetics
000001483 650_2 $$2MeSH$$aMembrane Potentials: radiation effects
000001483 650_2 $$2MeSH$$aMice
000001483 650_2 $$2MeSH$$aMice, Inbred C57BL
000001483 650_2 $$2MeSH$$aMice, Knockout
000001483 650_2 $$2MeSH$$aNeurons: metabolism
000001483 650_2 $$2MeSH$$aNeurons: radiation effects
000001483 650_2 $$2MeSH$$aOrgan Culture Techniques
000001483 650_2 $$2MeSH$$aPatch-Clamp Techniques
000001483 650_2 $$2MeSH$$aPhotic Stimulation
000001483 650_2 $$2MeSH$$aPhotoreceptor Cells, Vertebrate: metabolism
000001483 650_2 $$2MeSH$$aPhotoreceptor Cells, Vertebrate: radiation effects
000001483 650_2 $$2MeSH$$aPotassium Channels: genetics
000001483 650_2 $$2MeSH$$aRetina: metabolism
000001483 650_2 $$2MeSH$$aRetina: radiation effects
000001483 650_2 $$2MeSH$$aRetinal Bipolar Cells: metabolism
000001483 650_2 $$2MeSH$$aRetinal Bipolar Cells: radiation effects
000001483 650_2 $$2MeSH$$aRetinal Ganglion Cells: metabolism
000001483 650_2 $$2MeSH$$aRetinal Ganglion Cells: radiation effects
000001483 650_2 $$2MeSH$$aSynaptic Transmission: genetics
000001483 650_2 $$2MeSH$$aSynaptic Transmission: radiation effects
000001483 650_2 $$2MeSH$$aVision, Ocular: genetics
000001483 650_7 $$00$$2NLM Chemicals$$aCyclic Nucleotide-Gated Cation Channels
000001483 650_7 $$00$$2NLM Chemicals$$aPotassium Channels
000001483 650_7 $$00$$2NLM Chemicals$$ahyperpolarization-activated cation channel
000001483 650_7 $$2WoSType$$aJ
000001483 7001_ $$0P:(DE-HGF)0$$aSeeliger, M.W.$$b1
000001483 7001_ $$0P:(DE-Juel1)VDB8456$$aThiel, F.$$b2$$uFZJ
000001483 7001_ $$0P:(DE-Juel1)VDB64411$$aMataruga, A.$$b3$$uFZJ
000001483 7001_ $$0P:(DE-Juel1)VDB728$$aKaupp, U. B.$$b4$$uFZJ
000001483 7001_ $$0P:(DE-HGF)0$$aFriedburg, C.$$b5
000001483 7001_ $$0P:(DE-HGF)0$$aTanimoto, N.$$b6
000001483 7001_ $$0P:(DE-Juel1)131939$$aMüller, F.$$b7$$uFZJ
000001483 773__ $$0PERI:(DE-600)2005178-5$$a10.1111/j.1460-9568.2008.06512.x$$gVol. 28, p. 2221 - 2230$$p2221 - 2230$$q28<2221 - 2230$$tEuropean journal of neuroscience$$v28$$x0953-816X$$y2008
000001483 8567_ $$uhttp://dx.doi.org/10.1111/j.1460-9568.2008.06512.x
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