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@ARTICLE{Knop:1483,
author = {Knop, G.C. and Seeliger, M.W. and Thiel, F. and Mataruga,
A. and Kaupp, U. B. and Friedburg, C. and Tanimoto, N. and
Müller, F.},
title = {{L}ight responses in the mouse retina are prolonged upon
targeted deletion of the {HCN}1 channel gene},
journal = {European journal of neuroscience},
volume = {28},
issn = {0953-816X},
address = {Oxford [u.a.]},
publisher = {Blackwell},
reportid = {PreJuSER-1483},
pages = {2221 - 2230},
year = {2008},
note = {We thank Dr Eric Kandel ( Columbia University, USA) for
providing the HCN1 knock-out line, Christoph Aretzweiler for
technical assistance in immunohistochemistry and genotyping
of HCN1 knock-out animals, Mechthilde Bruns for help in cell
culture, and Dr Wolfgang Bonigk for the cDNA of murine HCN1.
M. W. Seeliger was supported by the Deutsche
Forschungsgemeinschaft ( DFG Se837/4-1 and Se837/5-1).},
abstract = {Hyperpolarization-activated and cyclic nucleotide-gated
(HCN) channels contribute to pacemaker activity, and
co-determine the integrative behaviour of neurons and shape
their response to synaptic stimulation. Four channel
isoforms, HCN1-4, have been described in mammals. Recent
studies showed particularly strong expression of HCN1
channels in rods and cones of the rat retina, suggesting
that HCN1 channels are involved in the shaping of light
responses in both types of photoreceptors. Therefore, the
loss of HCN1 channels should lead to pronounced changes in
light-induced electrical responses under both scotopic and
photopic conditions. This was tested using a mouse
transgenic approach. We used immunohistochemistry and
patch-clamp recording to study the distribution of HCN1
channels in the mouse retina. HCN1 channels were strongly
expressed in rod and cone photoreceptors, as well as in some
bipolar, amacrine and ganglion cell types. In
electroretinograms (ERGs) from animals in which the HCN1
channel gene had been knocked out, the b-wave amplitudes
were unaltered (scotopic conditions) or somewhat reduced
(photopic conditions), whereas the duration of both scotopic
and photopic ERG responses was strikingly prolonged. Our
data suggest that in visual information processing,
shortening and shaping of light responses by activation of
HCN1 at the level of the photoreceptors is an important step
in both scotopic and photopic pathways.},
keywords = {Amacrine Cells: metabolism / Amacrine Cells: radiation
effects / Animals / Cyclic Nucleotide-Gated Cation Channels:
genetics / Electroretinography / Immunohistochemistry /
Membrane Potentials: genetics / Membrane Potentials:
radiation effects / Mice / Mice, Inbred C57BL / Mice,
Knockout / Neurons: metabolism / Neurons: radiation effects
/ Organ Culture Techniques / Patch-Clamp Techniques / Photic
Stimulation / Photoreceptor Cells, Vertebrate: metabolism /
Photoreceptor Cells, Vertebrate: radiation effects /
Potassium Channels: genetics / Retina: metabolism / Retina:
radiation effects / Retinal Bipolar Cells: metabolism /
Retinal Bipolar Cells: radiation effects / Retinal Ganglion
Cells: metabolism / Retinal Ganglion Cells: radiation
effects / Synaptic Transmission: genetics / Synaptic
Transmission: radiation effects / Vision, Ocular: genetics /
Cyclic Nucleotide-Gated Cation Channels (NLM Chemicals) /
Potassium Channels (NLM Chemicals) /
hyperpolarization-activated cation channel (NLM Chemicals) /
J (WoSType)},
cin = {INB-1},
ddc = {610},
cid = {I:(DE-Juel1)VDB804},
pnm = {Funktion und Dysfunktion des Nervensystems},
pid = {G:(DE-Juel1)FUEK409},
shelfmark = {Neurosciences},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:19019198},
UT = {WOS:000261184800006},
doi = {10.1111/j.1460-9568.2008.06512.x},
url = {https://juser.fz-juelich.de/record/1483},
}