Hauptseite > Publikationsdatenbank > Light responses in the mouse retina are prolonged upon targeted deletion of the HCN1 channel gene > print

001     1483
005     20200423202401.0
024 7 _ |2 pmid
|a pmid:19019198
024 7 _ |2 DOI
|a 10.1111/j.1460-9568.2008.06512.x
024 7 _ |2 WOS
|a WOS:000261184800006
024 7 _ |2 Handle
|a 2128/4689
037 _ _ |a PreJuSER-1483
041 _ _ |a eng
082 _ _ |a 610
084 _ _ |2 WoS
|a Neurosciences
100 1 _ |0 P:(DE-Juel1)VDB79698
|a Knop, G.C.
|b 0
|u FZJ
245 _ _ |a Light responses in the mouse retina are prolonged upon targeted deletion of the HCN1 channel gene
260 _ _ |a Oxford [u.a.]
|b Blackwell
|c 2008
300 _ _ |a 2221 - 2230
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
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336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |0 1951
|a European Journal of Neuroscience
|v 28
|x 0953-816X
500 _ _ |a We thank Dr Eric Kandel ( Columbia University, USA) for providing the HCN1 knock-out line, Christoph Aretzweiler for technical assistance in immunohistochemistry and genotyping of HCN1 knock-out animals, Mechthilde Bruns for help in cell culture, and Dr Wolfgang Bonigk for the cDNA of murine HCN1. M. W. Seeliger was supported by the Deutsche Forschungsgemeinschaft ( DFG Se837/4-1 and Se837/5-1).
520 _ _ |a Hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels contribute to pacemaker activity, and co-determine the integrative behaviour of neurons and shape their response to synaptic stimulation. Four channel isoforms, HCN1-4, have been described in mammals. Recent studies showed particularly strong expression of HCN1 channels in rods and cones of the rat retina, suggesting that HCN1 channels are involved in the shaping of light responses in both types of photoreceptors. Therefore, the loss of HCN1 channels should lead to pronounced changes in light-induced electrical responses under both scotopic and photopic conditions. This was tested using a mouse transgenic approach. We used immunohistochemistry and patch-clamp recording to study the distribution of HCN1 channels in the mouse retina. HCN1 channels were strongly expressed in rod and cone photoreceptors, as well as in some bipolar, amacrine and ganglion cell types. In electroretinograms (ERGs) from animals in which the HCN1 channel gene had been knocked out, the b-wave amplitudes were unaltered (scotopic conditions) or somewhat reduced (photopic conditions), whereas the duration of both scotopic and photopic ERG responses was strikingly prolonged. Our data suggest that in visual information processing, shortening and shaping of light responses by activation of HCN1 at the level of the photoreceptors is an important step in both scotopic and photopic pathways.
536 _ _ |0 G:(DE-Juel1)FUEK409
|2 G:(DE-HGF)
|a Funktion und Dysfunktion des Nervensystems
|c P33
|x 0
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Amacrine Cells: metabolism
650 _ 2 |2 MeSH
|a Amacrine Cells: radiation effects
650 _ 2 |2 MeSH
|a Animals
650 _ 2 |2 MeSH
|a Cyclic Nucleotide-Gated Cation Channels: genetics
650 _ 2 |2 MeSH
|a Electroretinography
650 _ 2 |2 MeSH
|a Immunohistochemistry
650 _ 2 |2 MeSH
|a Membrane Potentials: genetics
650 _ 2 |2 MeSH
|a Membrane Potentials: radiation effects
650 _ 2 |2 MeSH
|a Mice
650 _ 2 |2 MeSH
|a Mice, Inbred C57BL
650 _ 2 |2 MeSH
|a Mice, Knockout
650 _ 2 |2 MeSH
|a Neurons: metabolism
650 _ 2 |2 MeSH
|a Neurons: radiation effects
650 _ 2 |2 MeSH
|a Organ Culture Techniques
650 _ 2 |2 MeSH
|a Patch-Clamp Techniques
650 _ 2 |2 MeSH
|a Photic Stimulation
650 _ 2 |2 MeSH
|a Photoreceptor Cells, Vertebrate: metabolism
650 _ 2 |2 MeSH
|a Photoreceptor Cells, Vertebrate: radiation effects
650 _ 2 |2 MeSH
|a Potassium Channels: genetics
650 _ 2 |2 MeSH
|a Retina: metabolism
650 _ 2 |2 MeSH
|a Retina: radiation effects
650 _ 2 |2 MeSH
|a Retinal Bipolar Cells: metabolism
650 _ 2 |2 MeSH
|a Retinal Bipolar Cells: radiation effects
650 _ 2 |2 MeSH
|a Retinal Ganglion Cells: metabolism
650 _ 2 |2 MeSH
|a Retinal Ganglion Cells: radiation effects
650 _ 2 |2 MeSH
|a Synaptic Transmission: genetics
650 _ 2 |2 MeSH
|a Synaptic Transmission: radiation effects
650 _ 2 |2 MeSH
|a Vision, Ocular: genetics
650 _ 7 |0 0
|2 NLM Chemicals
|a Cyclic Nucleotide-Gated Cation Channels
650 _ 7 |0 0
|2 NLM Chemicals
|a Potassium Channels
650 _ 7 |0 0
|2 NLM Chemicals
|a hyperpolarization-activated cation channel
650 _ 7 |2 WoSType
|a J
653 2 0 |2 Author
|a electrophysiology
653 2 0 |2 Author
|a electroretinography
653 2 0 |2 Author
|a ERG
653 2 0 |2 Author
|a HCN channels
653 2 0 |2 Author
|a I-h
653 2 0 |2 Author
|a retina
700 1 _ |0 P:(DE-HGF)0
|a Seeliger, M.W.
|b 1
700 1 _ |0 P:(DE-Juel1)VDB8456
|a Thiel, F.
|b 2
|u FZJ
700 1 _ |0 P:(DE-Juel1)VDB64411
|a Mataruga, A.
|b 3
|u FZJ
700 1 _ |0 P:(DE-Juel1)VDB728
|a Kaupp, U. B.
|b 4
|u FZJ
700 1 _ |0 P:(DE-HGF)0
|a Friedburg, C.
|b 5
700 1 _ |0 P:(DE-HGF)0
|a Tanimoto, N.
|b 6
700 1 _ |0 P:(DE-Juel1)131939
|a Müller, F.
|b 7
|u FZJ
773 _ _ |0 PERI:(DE-600)2005178-5
|a 10.1111/j.1460-9568.2008.06512.x
|g Vol. 28, p. 2221 - 2230
|p 2221 - 2230
|q 28<2221 - 2230
|t European journal of neuroscience
|v 28
|x 0953-816X
|y 2008
856 7 _ |u http://dx.doi.org/10.1111/j.1460-9568.2008.06512.x
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