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@ARTICLE{Wissel:1499,
author = {Wissel, H. and Mayr, C. and Lücke, A.},
title = {{A} new approach for the isolation of cellulose from
aquatic plant tissue and freshwater sediments fro stable
isotope analysis},
journal = {Organic geochemistry},
volume = {39},
issn = {0146-6380},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {PreJuSER-1499},
pages = {1545 - 1561},
year = {2008},
note = {We are indebted to Mrs. A. Richter for IR spectroscopic
analysis. D. Enters provided sediments from the Sacrower
See. Determination of aquatic plants by B. Messyasz is
gratefully acknowledged. We thank T.W.D. Edwards and P.
Finch for valuable comments. Financial support was provided
by the German Federal Ministry of Education and Research in
the framework of the German Climate Research Programme
DEKLIM (Grant 01 LD 0001).},
abstract = {Isotope studies of the cellulose of plant tissue and
sediments are increasingly important in ecological and
palaeoenvironmental studies. The reliability of analytical
results in these investigations depends on the ability to
separate pure cellulose from the organic and inorganic
matrix. This study evaluates the performance of a new
approach for the isolation of cellulose based on wet
oxidation and cellulose dissolution in cuprammonium solution
(CUAM) with respect to conventional techniques using solely
wet oxidation or wet oxidation in combination with density
separation. All the methods led to identical changes in
isotopic composition of standard cellulose powder samples,
providing evidence that CUAM-treated cellulose is
isotopically indistinguishable from cellulose treated with
conventional methods. The performance of CUAM with aquatic
plant tissue was at least equal to those of the other
methods used and in numerous cases achieved better results
in terms of cellulose purification and precision of the
isotope signal. Compared to conventional methods,
contamination of several samples with small amounts of
minerogenic matter and biogenic opal could be completely
removed from the extraction residue with CUAM. While
conventional methods failed to result in isolation of
cellulose from two typical fine-grained lacustrine
sediments, extraction residues from CUAM treatments revealed
infrared (IR) spectra resembling those of standard cellulose
powder, without evidence of minerogenic matter, biogenic
opal, chitin or refractory organic matter. A series of
sedimentary materials ranging from soil, to sediment trap
and sediment core material were extracted with CUAM. IR
spectra of all materials tested correspond to cellulose, and
carbon and oxygen isotope compositions of the extracted
cellulose fractions agreed with previous knowledge about the
samples. Our results prove that CUAM is a reliable method
that yields clean and pure cellulose for high quality
isotope analysis from plant tissue and different types of
sediments alike. (C) 2008 Elsevier Ltd. All rights
reserved.},
keywords = {J (WoSType)},
cin = {ICG-5},
ddc = {540},
cid = {I:(DE-Juel1)VDB143},
pnm = {Geosysteme - Erde im Wandel},
pid = {G:(DE-Juel1)FUEK405},
shelfmark = {Geochemistry $\&$ Geophysics},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000260948800006},
doi = {10.1016/j.orggeochem.2008.07.014},
url = {https://juser.fz-juelich.de/record/1499},
}