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000150201 1001_ $$0P:(DE-Juel1)132005$$aHänel, Karen$$b0$$ufzj
000150201 245__ $$aExpression and purification of soluble HIV-2 viral protein R (Vpr) using a sandwich-fusion protein strategy
000150201 260__ $$aOrlando, Fla.$$bAcademic Press$$c2014
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000150201 520__ $$aViral accessory proteins of the human immunodeficiency virus (HIV), including virus protein R (Vpr), are crucial for the efficient replication of the virus in the host organism. While functional data are available for HIV-1 Vpr, there is a paucity of data describing the function and structure of HIV-2 Vpr. In this report, the construction of a His6-MBP-intein1-Vpr-intein2-Cyt b5-His6 fusion protein is presented. Unlike previous research efforts where only microgram quantities of HIV-1 Vpr could be produced, this construct enabled soluble milligram yields via an Escherichia coli over-expression system. Straightforward protein purification of HIV-2 Vpr was achieved by standard chromatography routines and autocatalytic intein cleavage. Preliminary structural studies by circular dichroism (CD) and NMR spectroscopy revealed that the protein is stable in the presence of micellar concentrations of the detergent DPC and adopts an α-helix secondary structure.
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000150201 7001_ $$0P:(DE-Juel1)132013$$aMöckel, Luis$$b1$$ufzj
000150201 7001_ $$0P:(DE-HGF)0$$aBrummel, Monika$$b2
000150201 7001_ $$0P:(DE-HGF)0$$aPeiris, Katja$$b3
000150201 7001_ $$0P:(DE-Juel1)132001$$aHartmann, Rudolf$$b4$$ufzj
000150201 7001_ $$0P:(DE-HGF)0$$aDingley, Andrew J.$$b5
000150201 7001_ $$0P:(DE-Juel1)132029$$aWillbold, Dieter$$b6$$eCorresponding author$$ufzj
000150201 7001_ $$0P:(DE-HGF)0$$aLoidl-Stahlhofen, Angelika$$b7
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