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@ARTICLE{Hnel:150201,
      author       = {Hänel, Karen and Möckel, Luis and Brummel, Monika and
                      Peiris, Katja and Hartmann, Rudolf and Dingley, Andrew J.
                      and Willbold, Dieter and Loidl-Stahlhofen, Angelika},
      title        = {{E}xpression and purification of soluble {HIV}-2 viral
                      protein {R} ({V}pr) using a sandwich-fusion protein
                      strategy},
      journal      = {Protein expression and purification},
      volume       = {95},
      issn         = {1046-5928},
      address      = {Orlando, Fla.},
      publisher    = {Academic Press},
      reportid     = {FZJ-2014-00280},
      pages        = {156-161},
      year         = {2014},
      abstract     = {Viral accessory proteins of the human immunodeficiency
                      virus (HIV), including virus protein R (Vpr), are crucial
                      for the efficient replication of the virus in the host
                      organism. While functional data are available for HIV-1 Vpr,
                      there is a paucity of data describing the function and
                      structure of HIV-2 Vpr. In this report, the construction of
                      a His6-MBP-intein1-Vpr-intein2-Cyt b5-His6 fusion protein is
                      presented. Unlike previous research efforts where only
                      microgram quantities of HIV-1 Vpr could be produced, this
                      construct enabled soluble milligram yields via an
                      Escherichia coli over-expression system. Straightforward
                      protein purification of HIV-2 Vpr was achieved by standard
                      chromatography routines and autocatalytic intein cleavage.
                      Preliminary structural studies by circular dichroism (CD)
                      and NMR spectroscopy revealed that the protein is stable in
                      the presence of micellar concentrations of the detergent DPC
                      and adopts an α-helix secondary structure.},
      cin          = {ICS-6},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {452 - Structural Biology (POF2-452)},
      pid          = {G:(DE-HGF)POF2-452},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:24380802},
      UT           = {WOS:000332192900022},
      doi          = {10.1016/j.pep.2013.12.007},
      url          = {https://juser.fz-juelich.de/record/150201},
}