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@PHDTHESIS{Braun:150270,
      author       = {Braun, Regina},
      title        = {{P}henotyping {N}annochloropsis gaditana under different
                      conditions in controlle photobioreactor in laboratory and
                      upscaled photobioreactors in greenhouse},
      volume       = {221},
      school       = {Universität Düsseldorf},
      type         = {Dissertation},
      address      = {Jülich},
      publisher    = {Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag},
      reportid     = {FZJ-2014-00344},
      isbn         = {978-3-89336-975-1},
      series       = {Schriften des Forschungszentrums Jülich Reihe Energie $\&$
                      Umwelt / Energy $\&$ Environment},
      pages        = {177 S.},
      year         = {2013},
      note         = {Online-Publikation; Dissertation, Universität Düsseldorf,
                      2013},
      abstract     = {Since resources of fossil fuels are limited, alternatives
                      for energy production need to be explored. Besides plants as
                      biomass and energy crops, the interest in microalgaehas been
                      increasing, as they can synthesize many valuable compounds
                      with a large application range, including transport fuels or
                      fish and animal feed, without competing with agricultural
                      food production for arable land. Moreover, microalgae can
                      utilize ue gas from industrial emissions and municipal
                      wastewater as a nutrient source. For economically viable
                      production of algae, however, it is necessary to explore the
                      maximal potential of algae by optimized cultivation
                      conditions and targeted genetic modifications based on the
                      knowledge about their physiology as well as regulatory
                      mechanisms of growth and metabolic processes, in addition to
                      technical improvement of photobioreactors (PBRs) and
                      downstream processes. Circadian clocks synchronize certain
                      physiological, metabolic and developmental processes of
                      organisms with specific phases of recurring changes in their
                      environment, e.g. day and night or seasons. In this study it
                      was investigated whether the circadian clock plays a role in
                      regulation of growth and chlorophyll accumulation in
                      $\textit{Nannochloropsis gaditana}$, an oleaginous marine
                      microalga that is considered as a potential feedstock for
                      biofuels and for which a draft genome sequence has been
                      published. Optical density (OD) of $\textit{N. gadinata}$
                      culture was monitored at 680 and 735 nm under 12 h/12 h or
                      18 h/6 h light-dark (LD) cycles and after switching to
                      continuous illumination (LL) in controlled PBRs in the
                      laboratory. In parallel, chlorophyll fluorescence was
                      measured to assess the quantum yield of photosystem (PS) II.
                      Furthermore, to test if red- or blue-light photoreceptors
                      are involved in clock entrainment in $\textit{N. gaditana}$,
                      some of the experiments were conducted by using only red or
                      blue light. Growth and chlorophyll accumulation were
                      confined to light periods in the LD cycles, increasing more
                      strongly in the first half than in the second half of the
                      light periods. After switching to continuous light, rhythmic
                      oscillations persisted (especially for OD$_{680}$) at least
                      in the first 24 h, with a 50\% [...]},
      keywords     = {Dissertation (GND)},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {242 - Sustainable Bioproduction (POF2-242)},
      pid          = {G:(DE-HGF)POF2-242},
      typ          = {PUB:(DE-HGF)3 / PUB:(DE-HGF)11},
      url          = {https://juser.fz-juelich.de/record/150270},
}