%0 Journal Article
%A Huck, Christina
%A Schiffels, Johannes
%A Herrera, Cony N.
%A Schelden, Maximilian
%A Selmer, Thorsten
%A Poghossian, Arshak
%A Baumann, Marcus E. M.
%A Wagner, Patrick
%A Schöning, Michael J.
%T Metabolic responses of Escherichia coli upon glucose pulses captured by a capacitive field-effect sensor.
%J Physica status solidi / A
%V 210
%N 5
%@ 1862-6300
%C Weinheim
%I Wiley-VCH
%M FZJ-2014-00654
%P 926 - 931
%D 2013
%X Living cells are complex biological systems transforming
%X metabolites taken up from the surrounding medium. Monitoring
%X the responses of such cells to certain substrate concentrations is
%X a challenging task and offers possibilities to gain insight into the
%X vitality of a community influenced by the growth environment.
%X Cell-based sensors represent a promising platform for monitoring
%X the metabolic activity and thus, the ‘‘welfare’’ of relevant
%X organisms. In the present study, metabolic responses of the
%X model bacterium Escherichia coli in suspension, layered onto a
%X capacitive field-effect structure, were examined to pulses of
%X glucose in the concentration range between 0.05 and 2 mM. It
%X was found that acidification of the surrounding medium takes
%X place immediately after glucose addition and follows Michaelis–
%X Menten kinetic behavior as a function of the glucose
%X concentration. In future, the presented setup can, therefore, be
%X used to study substrate specificities on the enzymatic level and
%X may as well be used to perform investigations of more complex
%X metabolic responses. Conclusions and perspectives highlighting
%X this system are discussed.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000319151900016
%R 10.1002/pssa.201200900
%U https://juser.fz-juelich.de/record/150605