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000150605 0247_ $$2doi$$a10.1002/pssa.201200900
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000150605 1001_ $$0P:(DE-Juel1)128688$$aHuck, Christina$$b0$$eCorresponding author$$ufzj
000150605 245__ $$aMetabolic responses of Escherichia coli upon glucose pulses captured by a capacitive field-effect sensor.
000150605 260__ $$aWeinheim$$bWiley-VCH$$c2013
000150605 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1390919891_7662
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000150605 500__ $$3POF3_Assignment on 2016-02-29
000150605 520__ $$aLiving cells are complex biological systems transforming
metabolites taken up from the surrounding medium. Monitoring
the responses of such cells to certain substrate concentrations is
a challenging task and offers possibilities to gain insight into the
vitality of a community influenced by the growth environment.
Cell-based sensors represent a promising platform for monitoring
the metabolic activity and thus, the ‘‘welfare’’ of relevant
organisms. In the present study, metabolic responses of the
model bacterium Escherichia coli in suspension, layered onto a
capacitive field-effect structure, were examined to pulses of
glucose in the concentration range between 0.05 and 2 mM. It
was found that acidification of the surrounding medium takes
place immediately after glucose addition and follows Michaelis–
Menten kinetic behavior as a function of the glucose
concentration. In future, the presented setup can, therefore, be
used to study substrate specificities on the enzymatic level and
may as well be used to perform investigations of more complex
metabolic responses. Conclusions and perspectives highlighting
this system are discussed.
000150605 536__ $$0G:(DE-HGF)POF2-423$$a423 - Sensorics and bioinspired systems (POF2-423)$$cPOF2-423$$fPOF II$$x0
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000150605 7001_ $$0P:(DE-HGF)0$$aSchiffels, Johannes$$b1
000150605 7001_ $$0P:(DE-HGF)0$$aHerrera, Cony N.$$b2
000150605 7001_ $$0P:(DE-HGF)0$$aSchelden, Maximilian$$b3
000150605 7001_ $$0P:(DE-HGF)0$$aSelmer, Thorsten$$b4
000150605 7001_ $$0P:(DE-Juel1)128717$$aPoghossian, Arshak$$b5$$ufzj
000150605 7001_ $$0P:(DE-HGF)0$$aBaumann, Marcus E. M.$$b6
000150605 7001_ $$0P:(DE-HGF)0$$aWagner, Patrick$$b7
000150605 7001_ $$0P:(DE-Juel1)128727$$aSchöning, Michael J.$$b8$$ufzj
000150605 773__ $$0PERI:(DE-600)1481091-8$$a10.1002/pssa.201200900$$gVol. 210, no. 5, p. 926 - 931$$n5$$p926 - 931$$tPhysica status solidi / A$$v210$$x1862-6300$$y2013
000150605 8564_ $$uhttps://juser.fz-juelich.de/record/150605/files/FZJ-2014-00654.pdf$$yRestricted$$zPublished final document.
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