TY  - JOUR
AU  - Huck, Christina
AU  - Schiffels, Johannes
AU  - Herrera, Cony N.
AU  - Schelden, Maximilian
AU  - Selmer, Thorsten
AU  - Poghossian, Arshak
AU  - Baumann, Marcus E. M.
AU  - Wagner, Patrick
AU  - Schöning, Michael J.
TI  - Metabolic responses of Escherichia coli upon glucose pulses captured by a capacitive field-effect sensor.
JO  - Physica status solidi / A
VL  - 210
IS  - 5
SN  - 1862-6300
CY  - Weinheim
PB  - Wiley-VCH
M1  - FZJ-2014-00654
SP  - 926 - 931
PY  - 2013
AB  - Living cells are complex biological systems transforming
AB  - metabolites taken up from the surrounding medium. Monitoring
AB  - the responses of such cells to certain substrate concentrations is
AB  - a challenging task and offers possibilities to gain insight into the
AB  - vitality of a community influenced by the growth environment.
AB  - Cell-based sensors represent a promising platform for monitoring
AB  - the metabolic activity and thus, the ‘‘welfare’’ of relevant
AB  - organisms. In the present study, metabolic responses of the
AB  - model bacterium Escherichia coli in suspension, layered onto a
AB  - capacitive field-effect structure, were examined to pulses of
AB  - glucose in the concentration range between 0.05 and 2 mM. It
AB  - was found that acidification of the surrounding medium takes
AB  - place immediately after glucose addition and follows Michaelis–
AB  - Menten kinetic behavior as a function of the glucose
AB  - concentration. In future, the presented setup can, therefore, be
AB  - used to study substrate specificities on the enzymatic level and
AB  - may as well be used to perform investigations of more complex
AB  - metabolic responses. Conclusions and perspectives highlighting
AB  - this system are discussed.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000319151900016
DO  - DOI:10.1002/pssa.201200900
UR  - https://juser.fz-juelich.de/record/150605
ER  -