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@ARTICLE{Huck:150605,
      author       = {Huck, Christina and Schiffels, Johannes and Herrera, Cony
                      N. and Schelden, Maximilian and Selmer, Thorsten and
                      Poghossian, Arshak and Baumann, Marcus E. M. and Wagner,
                      Patrick and Schöning, Michael J.},
      title        = {{M}etabolic responses of {E}scherichia coli upon glucose
                      pulses captured by a capacitive field-effect sensor.},
      journal      = {Physica status solidi / A},
      volume       = {210},
      number       = {5},
      issn         = {1862-6300},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {FZJ-2014-00654},
      pages        = {926 - 931},
      year         = {2013},
      abstract     = {Living cells are complex biological systems transforming
                      metabolites taken up from the surrounding medium. Monitoring
                      the responses of such cells to certain substrate
                      concentrations is a challenging task and offers
                      possibilities to gain insight into the vitality of a
                      community influenced by the growth environment. Cell-based
                      sensors represent a promising platform for monitoring the
                      metabolic activity and thus, the ‘‘welfare’’ of
                      relevant organisms. In the present study, metabolic
                      responses of the model bacterium Escherichia coli in
                      suspension, layered onto a capacitive field-effect
                      structure, were examined to pulses of glucose in the
                      concentration range between 0.05 and 2 mM. It was found that
                      acidification of the surrounding medium takes place
                      immediately after glucose addition and follows Michaelis–
                      Menten kinetic behavior as a function of the glucose
                      concentration. In future, the presented setup can,
                      therefore, be used to study substrate specificities on the
                      enzymatic level and may as well be used to perform
                      investigations of more complex metabolic responses.
                      Conclusions and perspectives highlighting this system are
                      discussed.},
      cin          = {PGI-8 / JARA-FIT / ICS-8},
      ddc          = {530},
      cid          = {I:(DE-Juel1)PGI-8-20110106 / $I:(DE-82)080009_20140620$ /
                      I:(DE-Juel1)ICS-8-20110106},
      pnm          = {423 - Sensorics and bioinspired systems (POF2-423) / 453 -
                      Physics of the Cell (POF2-453)},
      pid          = {G:(DE-HGF)POF2-423 / G:(DE-HGF)POF2-453},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000319151900016},
      doi          = {10.1002/pssa.201200900},
      url          = {https://juser.fz-juelich.de/record/150605},
}