000151178 001__ 151178
000151178 005__ 20250129092427.0
000151178 0247_ $$2doi$$a10.1038/nature09750
000151178 0247_ $$2ISSN$$a1476-4687
000151178 0247_ $$2ISSN$$a0028-0836
000151178 0247_ $$2WOS$$aWOS:000286886400036
000151178 0247_ $$2altmetric$$aaltmetric:130809
000151178 0247_ $$2pmid$$apmid:21293373
000151178 037__ $$aFZJ-2014-01177
000151178 082__ $$a070
000151178 1001_ $$0P:(DE-HGF)0$$aChapman, Henry N.$$b0$$eCorresponding author
000151178 245__ $$aFemtosecond X-ray protein nanocrystallography
000151178 260__ $$aLondon [u.a.]$$bNature Publising Group$$c2011
000151178 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1406899605_27347
000151178 3367_ $$2DataCite$$aOutput Types/Journal article
000151178 3367_ $$00$$2EndNote$$aJournal Article
000151178 3367_ $$2BibTeX$$aARTICLE
000151178 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000151178 3367_ $$2DRIVER$$aarticle
000151178 520__ $$aX-ray crystallography provides the vast majority of macromolecular structures, but the success of the method relies on growing crystals of sufficient size. In conventional measurements, the necessary increase in X-ray dose to record data from crystals that are too small leads to extensive damage before a diffraction signal can be recorded1, 2, 3. It is particularly challenging to obtain large, well-diffracting crystals of membrane proteins, for which fewer than 300 unique structures have been determined despite their importance in all living cells. Here we present a method for structure determination where single-crystal X-ray diffraction ‘snapshots’ are collected from a fully hydrated stream of nanocrystals using femtosecond pulses from a hard-X-ray free-electron laser, the Linac Coherent Light Source4. We prove this concept with nanocrystals of photosystem I, one of the largest membrane protein complexes5. More than 3,000,000 diffraction patterns were collected in this study, and a three-dimensional data set was assembled from individual photosystem I nanocrystals (~200 nm to 2 μm in size). We mitigate the problem of radiation damage in crystallography by using pulses briefer than the timescale of most damage processes6. This offers a new approach to structure determination of macromolecules that do not yield crystals of sufficient size for studies using conventional radiation sources or are particularly sensitive to radiation damage.
000151178 536__ $$0G:(DE-HGF)POF2-531$$a531 - Hadron Structure and Dynamics (HSD) (POF2-531)$$cPOF2-531$$fPOF II$$x0
000151178 588__ $$aDataset connected to CrossRef, juser.fz-juelich.de
000151178 7001_ $$0P:(DE-HGF)0$$aFromme, Petra$$b1
000151178 7001_ $$0P:(DE-HGF)0$$aBarty, Anton$$b2
000151178 7001_ $$0P:(DE-HGF)0$$aWhite, Thomas A.$$b3
000151178 7001_ $$0P:(DE-HGF)0$$aKirian, Richard A.$$b4
000151178 7001_ $$0P:(DE-HGF)0$$aAquila, Andrew$$b5
000151178 7001_ $$0P:(DE-HGF)0$$aHunter, Mark S.$$b6
000151178 7001_ $$0P:(DE-HGF)0$$aSchulz, Joachim$$b7
000151178 7001_ $$0P:(DE-HGF)0$$aDePonte, Daniel P.$$b8
000151178 7001_ $$0P:(DE-HGF)0$$aWeierstall, Uwe$$b9
000151178 7001_ $$0P:(DE-HGF)0$$aDoak, R. Bruce$$b10
000151178 7001_ $$0P:(DE-HGF)0$$aMaia, Filipe R. N. C.$$b11
000151178 7001_ $$0P:(DE-HGF)0$$aMartin, Andrew V.$$b12
000151178 7001_ $$0P:(DE-HGF)0$$aSchlichting, Ilme$$b13
000151178 7001_ $$0P:(DE-HGF)0$$aLomb, Lukas$$b14
000151178 7001_ $$0P:(DE-HGF)0$$aCoppola, Nicola$$b15
000151178 7001_ $$0P:(DE-HGF)0$$aShoeman, Robert L.$$b16
000151178 7001_ $$0P:(DE-HGF)0$$aEpp, Sascha W.$$b17
000151178 7001_ $$0P:(DE-HGF)0$$aHartmann, Robert$$b18
000151178 7001_ $$0P:(DE-HGF)0$$aRolles, Daniel$$b19
000151178 7001_ $$0P:(DE-HGF)0$$aRudenko, Artem$$b20
000151178 7001_ $$0P:(DE-HGF)0$$aFoucar, Lutz$$b21
000151178 7001_ $$0P:(DE-HGF)0$$aKimmel, Nils$$b22
000151178 7001_ $$0P:(DE-HGF)0$$aWeidenspointner, Georg$$b23
000151178 7001_ $$0P:(DE-HGF)0$$aHoll, Peter$$b24
000151178 7001_ $$0P:(DE-HGF)0$$aLiang, Mengning$$b25
000151178 7001_ $$0P:(DE-HGF)0$$aBarthelmess, Miriam$$b26
000151178 7001_ $$0P:(DE-HGF)0$$aCaleman, Carl$$b27
000151178 7001_ $$0P:(DE-HGF)0$$aBoutet, Sébastien$$b28
000151178 7001_ $$0P:(DE-HGF)0$$aBogan, Michael J.$$b29
000151178 7001_ $$0P:(DE-HGF)0$$aKrzywinski, Jacek$$b30
000151178 7001_ $$0P:(DE-HGF)0$$aBostedt, Christoph$$b31
000151178 7001_ $$0P:(DE-HGF)0$$aBajt, Saša$$b32
000151178 7001_ $$0P:(DE-HGF)0$$aGumprecht, Lars$$b33
000151178 7001_ $$0P:(DE-HGF)0$$aRudek, Benedikt$$b34
000151178 7001_ $$0P:(DE-HGF)0$$aErk, Benjamin$$b35
000151178 7001_ $$0P:(DE-HGF)0$$aSchmidt, Carlo$$b36
000151178 7001_ $$0P:(DE-HGF)0$$aHömke, André$$b37
000151178 7001_ $$0P:(DE-HGF)0$$aReich, Christian$$b38
000151178 7001_ $$0P:(DE-HGF)0$$aPietschner, Daniel$$b39
000151178 7001_ $$0P:(DE-HGF)0$$aStrüder, Lothar$$b40
000151178 7001_ $$0P:(DE-HGF)0$$aHauser, Günter$$b41
000151178 7001_ $$0P:(DE-Juel1)133892$$aGorke, Hubert$$b42
000151178 7001_ $$0P:(DE-HGF)0$$aUllrich, Joachim$$b43
000151178 7001_ $$0P:(DE-HGF)0$$aHerrmann, Sven$$b44
000151178 7001_ $$0P:(DE-HGF)0$$aSchaller, Gerhard$$b45
000151178 7001_ $$0P:(DE-HGF)0$$aSchopper, Florian$$b46
000151178 7001_ $$0P:(DE-HGF)0$$aSoltau, Heike$$b47
000151178 7001_ $$0P:(DE-HGF)0$$aKühnel, Kai-Uwe$$b48
000151178 7001_ $$0P:(DE-HGF)0$$aMesserschmidt, Marc$$b49
000151178 7001_ $$0P:(DE-HGF)0$$aBozek, John D.$$b50
000151178 7001_ $$0P:(DE-HGF)0$$aHau-Riege, Stefan P.$$b51
000151178 7001_ $$0P:(DE-HGF)0$$aFrank, Matthias$$b52
000151178 7001_ $$0P:(DE-HGF)0$$aHampton, Christina Y.$$b53
000151178 7001_ $$0P:(DE-HGF)0$$aSierra, Raymond G.$$b54
000151178 7001_ $$0P:(DE-HGF)0$$aStarodub, Dmitri$$b55
000151178 7001_ $$0P:(DE-HGF)0$$aWilliams, Garth J.$$b56
000151178 7001_ $$0P:(DE-HGF)0$$aHajdu, Janos$$b57
000151178 7001_ $$0P:(DE-HGF)0$$aTimneanu, Nicusor$$b58
000151178 7001_ $$0P:(DE-HGF)0$$aSeibert, M. Marvin$$b59
000151178 7001_ $$0P:(DE-HGF)0$$aAndreasson, Jakob$$b60
000151178 7001_ $$0P:(DE-HGF)0$$aRocker, Andrea$$b61
000151178 7001_ $$0P:(DE-HGF)0$$aJönsson, Olof$$b62
000151178 7001_ $$0P:(DE-HGF)0$$aSvenda, Martin$$b63
000151178 7001_ $$0P:(DE-HGF)0$$aStern, Stephan$$b64
000151178 7001_ $$0P:(DE-HGF)0$$aNass, Karol$$b65
000151178 7001_ $$0P:(DE-HGF)0$$aAndritschke, Robert$$b66
000151178 7001_ $$0P:(DE-HGF)0$$aSchröter, Claus-Dieter$$b67
000151178 7001_ $$0P:(DE-HGF)0$$aKrasniqi, Faton$$b68
000151178 7001_ $$0P:(DE-HGF)0$$aBott, Mario$$b69
000151178 7001_ $$0P:(DE-HGF)0$$aSchmidt, Kevin E.$$b70
000151178 7001_ $$0P:(DE-HGF)0$$aWang, Xiaoyu$$b71
000151178 7001_ $$0P:(DE-HGF)0$$aGrotjohann, Ingo$$b72
000151178 7001_ $$0P:(DE-HGF)0$$aHolton, James M.$$b73
000151178 7001_ $$0P:(DE-HGF)0$$aBarends, Thomas R. M.$$b74
000151178 7001_ $$0P:(DE-HGF)0$$aNeutze, Richard$$b75
000151178 7001_ $$0P:(DE-HGF)0$$aMarchesini, Stefano$$b76
000151178 7001_ $$0P:(DE-HGF)0$$aFromme, Raimund$$b77
000151178 7001_ $$0P:(DE-HGF)0$$aSchorb, Sebastian$$b78
000151178 7001_ $$0P:(DE-HGF)0$$aRupp, Daniela$$b79
000151178 7001_ $$0P:(DE-HGF)0$$aAdolph, Marcus$$b80
000151178 7001_ $$0P:(DE-HGF)0$$aGorkhover, Tais$$b81
000151178 7001_ $$0P:(DE-HGF)0$$aAndersson, Inger$$b82
000151178 7001_ $$0P:(DE-HGF)0$$aHirsemann, Helmut$$b83
000151178 7001_ $$0P:(DE-HGF)0$$aPotdevin, Guillaume$$b84
000151178 7001_ $$0P:(DE-HGF)0$$aGraafsma, Heinz$$b85
000151178 7001_ $$0P:(DE-HGF)0$$aNilsson, Björn$$b86
000151178 7001_ $$0P:(DE-HGF)0$$aSpence, John C. H.$$b87
000151178 773__ $$0PERI:(DE-600)1413423-8$$a10.1038/nature09750$$gVol. 470, no. 7332, p. 73 - 77$$n7332$$p73 - 77$$tNature <London>$$v470$$x1476-4687$$y2011
000151178 8564_ $$uhttps://juser.fz-juelich.de/record/151178/files/FZJ-2014-01177.pdf$$yRestricted
000151178 909CO $$ooai:juser.fz-juelich.de:151178$$pVDB
000151178 9101_ $$0I:(DE-Juel1)ZEA-2-20090406$$6P:(DE-Juel1)133892$$aZentralinstitut für Elektronik$$b42$$kZEA-2
000151178 9132_ $$0G:(DE-HGF)POF3-531$$1G:(DE-HGF)POF3-530$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lScience and Technology of Nanosystems$$vCondensed Matter and Molecular Building Blocks$$x0
000151178 9131_ $$0G:(DE-HGF)POF2-531$$1G:(DE-HGF)POF2-530$$2G:(DE-HGF)POF2-500$$3G:(DE-HGF)POF2$$4G:(DE-HGF)POF$$aDE-HGF$$bStruktur der Materie$$lPhysik der Hadronen und Kerne$$vHadron Structure and Dynamics (HSD)$$x0
000151178 9141_ $$y2014
000151178 915__ $$0StatID:(DE-HGF)0010$$2StatID$$aJCR/ISI refereed
000151178 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR
000151178 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000151178 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000151178 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000151178 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000151178 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000151178 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000151178 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000151178 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz
000151178 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences
000151178 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record
000151178 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000151178 915__ $$0StatID:(DE-HGF)1060$$2StatID$$aDBCoverage$$bCurrent Contents - Agriculture, Biology and Environmental Sciences
000151178 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences
000151178 920__ $$lyes
000151178 9201_ $$0I:(DE-Juel1)ZEA-2-20090406$$kZEA-2$$lZentralinstitut für Elektronik$$x0
000151178 980__ $$ajournal
000151178 980__ $$aVDB
000151178 980__ $$aI:(DE-Juel1)ZEA-2-20090406
000151178 980__ $$aUNRESTRICTED
000151178 981__ $$aI:(DE-Juel1)PGI-4-20110106