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| 001 | 151179 | ||
| 005 | 20250129092449.0 | ||
| 024 | 7 | _ | |a 10.1038/nature09748 |2 doi |
| 024 | 7 | _ | |a 1476-4687 |2 ISSN |
| 024 | 7 | _ | |a 0028-0836 |2 ISSN |
| 024 | 7 | _ | |a WOS:000286886400037 |2 WOS |
| 024 | 7 | _ | |a altmetric:100189 |2 altmetric |
| 024 | 7 | _ | |a pmid:21293374 |2 pmid |
| 037 | _ | _ | |a FZJ-2014-01178 |
| 082 | _ | _ | |a 070 |
| 100 | 1 | _ | |a Seibert, M. Marvin |0 P:(DE-HGF)0 |b 0 |e Corresponding author |
| 245 | _ | _ | |a Single mimivirus particles intercepted and imaged with an X-ray laser |
| 260 | _ | _ | |a London [u.a.] |c 2011 |b Nature Publising Group78092 |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1406899731_27352 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 520 | _ | _ | |a X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions1, 2, 3, 4. Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma1. The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval2. Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a non-crystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source5. Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000 K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies. |
| 536 | _ | _ | |a 531 - Hadron Structure and Dynamics (HSD) (POF2-531) |0 G:(DE-HGF)POF2-531 |c POF2-531 |f POF II |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef, juser.fz-juelich.de |
| 700 | 1 | _ | |a Ekeberg, Tomas |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Maia, Filipe R. N. C. |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Svenda, Martin |0 P:(DE-HGF)0 |b 3 |
| 700 | 1 | _ | |a Andreasson, Jakob |0 P:(DE-HGF)0 |b 4 |
| 700 | 1 | _ | |a Jönsson, Olof |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a Odić, Duško |0 P:(DE-HGF)0 |b 6 |
| 700 | 1 | _ | |a Iwan, Bianca |0 P:(DE-HGF)0 |b 7 |
| 700 | 1 | _ | |a Rocker, Andrea |0 P:(DE-HGF)0 |b 8 |
| 700 | 1 | _ | |a Westphal, Daniel |0 P:(DE-HGF)0 |b 9 |
| 700 | 1 | _ | |a Hantke, Max |0 P:(DE-HGF)0 |b 10 |
| 700 | 1 | _ | |a DePonte, Daniel P. |0 P:(DE-HGF)0 |b 11 |
| 700 | 1 | _ | |a Barty, Anton |0 P:(DE-HGF)0 |b 12 |
| 700 | 1 | _ | |a Schulz, Joachim |0 P:(DE-HGF)0 |b 13 |
| 700 | 1 | _ | |a Gumprecht, Lars |0 P:(DE-HGF)0 |b 14 |
| 700 | 1 | _ | |a Coppola, Nicola |0 P:(DE-HGF)0 |b 15 |
| 700 | 1 | _ | |a Aquila, Andrew |0 P:(DE-HGF)0 |b 16 |
| 700 | 1 | _ | |a Liang, Mengning |0 P:(DE-HGF)0 |b 17 |
| 700 | 1 | _ | |a White, Thomas A. |0 P:(DE-HGF)0 |b 18 |
| 700 | 1 | _ | |a Martin, Andrew |0 P:(DE-HGF)0 |b 19 |
| 700 | 1 | _ | |a Caleman, Carl |0 P:(DE-HGF)0 |b 20 |
| 700 | 1 | _ | |a Stern, Stephan |0 P:(DE-HGF)0 |b 21 |
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| 700 | 1 | _ | |a Bostedt, Christoph |0 P:(DE-HGF)0 |b 25 |
| 700 | 1 | _ | |a Bozek, John D. |0 P:(DE-HGF)0 |b 26 |
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| 773 | _ | _ | |a 10.1038/nature09748 |g Vol. 470, no. 7332, p. 78 - 81 |0 PERI:(DE-600)1413423-8 |n 7332 |p 78 - 81 |t Nature |v 470 |y 2011 |x 1476-4687 |
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