000015171 001__ 15171
000015171 005__ 20180208232840.0
000015171 0247_ $$2pmid$$apmid:21058101
000015171 0247_ $$2DOI$$a10.1080/10256016.2010.528840
000015171 0247_ $$2WOS$$aWOS:000285149100002
000015171 0247_ $$2ISSN$$a1477-2639
000015171 037__ $$aPreJuSER-15171
000015171 041__ $$aeng
000015171 082__ $$a540
000015171 084__ $$2WoS$$aChemistry, Inorganic & Nuclear
000015171 084__ $$2WoS$$aEnvironmental Sciences
000015171 1001_ $$0P:(DE-HGF)0$$aMeyer, A.$$b0
000015171 245__ $$aA new 15N tracer method to determine N turnover and denitrification of Pseudomonas stutzeri
000015171 260__ $$aLondon [u.a.]$$bTaylor and Francis$$c2010
000015171 300__ $$a409 - 421
000015171 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000015171 3367_ $$2DataCite$$aOutput Types/Journal article
000015171 3367_ $$00$$2EndNote$$aJournal Article
000015171 3367_ $$2BibTeX$$aARTICLE
000015171 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000015171 3367_ $$2DRIVER$$aarticle
000015171 440_0 $$02918$$aIsotopes in Environmental and Health Studies$$v46$$x1025-6016$$y4
000015171 500__ $$aThis work was funded by the Deutsche Forschungsgemeinschaft (DFG) within the Beech Research Group (FOR 788), the EU-funded integrated project NitroEurope and by the Joint Helmholtz/Chinese Academy of Sciences Laboratory ENTRANCE.
000015171 520__ $$aAlthough denitrification is one of the key processes of ecosystem N turnover, the understanding of the regulation of the denitrification pathway is still limited due to the lack of feasible methods for the quantification of N₂ formation. Based on the previously developed isotope pairing method, we present a new in vitro ¹⁵N tracer method for the quantification of N₂ released from denitrification by bacterial cultures. The application of the new method was enabled by replacing the background air in the sample flasks with a gas mixture of He and O₂ with an approximately 50-fold reduced N₂ background (1.7% v/v), allowing for a direct and sensitive quantification of N₂ formation with isotope-ratio mass spectrometry after ¹⁵N-labelling on the one hand, but leaving the method relatively insensitive to intrusion of ambient N₂ on the other hand. The method was tested on bacterial cultures of Pseudomonas stutzeri grown at different oxygen levels. Additionally, NO and N₂O formation were determined with a chemoluminescence analyser and a gas chromatograph, respectively. Following labelling with ¹⁵N-ammonium and ¹⁵N-nitrate, it could be shown that P. stutzeri used ammonium preferably for biomass build-up, and nitrate preferably as electron acceptor. Between 84-107% of the total available N could be recovered. Due to the high sensitivity of the new method only low levels of ¹⁵N tracer were necessary, minimising substrate-induced effects and making this method also an appropriate tool for the use on soil cores. By that it offers a new method for studying denitrification in terrestrial ecosystems.
000015171 536__ $$0G:(DE-Juel1)FUEK407$$2G:(DE-HGF)$$aTerrestrische Umwelt$$cP24$$x0
000015171 588__ $$aDataset connected to Web of Science, Pubmed
000015171 65320 $$2Author$$adenitrification
000015171 65320 $$2Author$$aisotope pairing
000015171 65320 $$2Author$$aPseudomonas stutzeri
000015171 65320 $$2Author$$anitrogen-15
000015171 65320 $$2Author$$aisotope ecology
000015171 65320 $$2Author$$atracer technique
000015171 650_2 $$2MeSH$$aDenitrification
000015171 650_2 $$2MeSH$$aNitrogen Isotopes: metabolism
000015171 650_2 $$2MeSH$$aPseudomonas stutzeri: metabolism
000015171 650_7 $$00$$2NLM Chemicals$$aNitrogen Isotopes
000015171 650_7 $$2WoSType$$aJ
000015171 7001_ $$0P:(DE-HGF)0$$aBergmann, J.$$b1
000015171 7001_ $$0P:(DE-HGF)0$$aButterbach-Bahl, K.$$b2
000015171 7001_ $$0P:(DE-Juel1)142357$$aBrüggemann, N.$$b3$$uFZJ
000015171 773__ $$0PERI:(DE-600)2100190-X$$a10.1080/10256016.2010.528840$$gVol. 46, p. 409 - 421$$p409 - 421$$q46<409 - 421$$tIsotopes in Environmental and Health Studies$$v46$$x1025-6016$$y2010
000015171 8567_ $$uhttp://dx.doi.org/10.1080/10256016.2010.528840
000015171 909CO $$ooai:juser.fz-juelich.de:15171$$pVDB
000015171 915__ $$0StatID:(DE-HGF)0010$$2StatID$$aJCR/ISI refereed
000015171 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR
000015171 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000015171 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000015171 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000015171 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000015171 9141_ $$aNachtrag$$y2010
000015171 9131_ $$0G:(DE-Juel1)FUEK407$$aDE-HGF$$bErde und Umwelt$$kP24$$lTerrestrische Umwelt$$vTerrestrische Umwelt$$x0
000015171 9201_ $$0I:(DE-Juel1)IBG-3-20101118$$gIBG$$kIBG-3$$lAgrosphäre$$x0
000015171 970__ $$aVDB:(DE-Juel1)127904
000015171 980__ $$aVDB
000015171 980__ $$aConvertedRecord
000015171 980__ $$ajournal
000015171 980__ $$aI:(DE-Juel1)IBG-3-20101118
000015171 980__ $$aUNRESTRICTED