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@ARTICLE{Meyer:15171,
      author       = {Meyer, A. and Bergmann, J. and Butterbach-Bahl, K. and
                      Brüggemann, N.},
      title        = {{A} new 15{N} tracer method to determine {N} turnover and
                      denitrification of {P}seudomonas stutzeri},
      journal      = {Isotopes in Environmental and Health Studies},
      volume       = {46},
      issn         = {1025-6016},
      address      = {London [u.a.]},
      publisher    = {Taylor and Francis},
      reportid     = {PreJuSER-15171},
      pages        = {409 - 421},
      year         = {2010},
      note         = {This work was funded by the Deutsche Forschungsgemeinschaft
                      (DFG) within the Beech Research Group (FOR 788), the
                      EU-funded integrated project NitroEurope and by the Joint
                      Helmholtz/Chinese Academy of Sciences Laboratory ENTRANCE.},
      abstract     = {Although denitrification is one of the key processes of
                      ecosystem N turnover, the understanding of the regulation of
                      the denitrification pathway is still limited due to the lack
                      of feasible methods for the quantification of N₂
                      formation. Based on the previously developed isotope pairing
                      method, we present a new in vitro ¹⁵N tracer method for
                      the quantification of N₂ released from denitrification by
                      bacterial cultures. The application of the new method was
                      enabled by replacing the background air in the sample flasks
                      with a gas mixture of He and O₂ with an approximately
                      50-fold reduced N₂ background $(1.7\%$ v/v), allowing for
                      a direct and sensitive quantification of N₂ formation with
                      isotope-ratio mass spectrometry after ¹⁵N-labelling on
                      the one hand, but leaving the method relatively insensitive
                      to intrusion of ambient N₂ on the other hand. The method
                      was tested on bacterial cultures of Pseudomonas stutzeri
                      grown at different oxygen levels. Additionally, NO and N₂O
                      formation were determined with a chemoluminescence analyser
                      and a gas chromatograph, respectively. Following labelling
                      with ¹⁵N-ammonium and ¹⁵N-nitrate, it could be shown
                      that P. stutzeri used ammonium preferably for biomass
                      build-up, and nitrate preferably as electron acceptor.
                      Between $84-107\%$ of the total available N could be
                      recovered. Due to the high sensitivity of the new method
                      only low levels of ¹⁵N tracer were necessary, minimising
                      substrate-induced effects and making this method also an
                      appropriate tool for the use on soil cores. By that it
                      offers a new method for studying denitrification in
                      terrestrial ecosystems.},
      keywords     = {Denitrification / Nitrogen Isotopes: metabolism /
                      Pseudomonas stutzeri: metabolism / Nitrogen Isotopes (NLM
                      Chemicals) / J (WoSType)},
      cin          = {IBG-3},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-3-20101118},
      pnm          = {Terrestrische Umwelt},
      pid          = {G:(DE-Juel1)FUEK407},
      shelfmark    = {Chemistry, Inorganic $\&$ Nuclear / Environmental Sciences},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:21058101},
      UT           = {WOS:000285149100002},
      doi          = {10.1080/10256016.2010.528840},
      url          = {https://juser.fz-juelich.de/record/15171},
}