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| Home > Publications database > A new 15N tracer method to determine N turnover and denitrification of Pseudomonas stutzeri > print |
| 001 | 15171 | ||
| 005 | 20180208232840.0 | ||
| 024 | 7 | _ | |2 pmid |a pmid:21058101 |
| 024 | 7 | _ | |2 DOI |a 10.1080/10256016.2010.528840 |
| 024 | 7 | _ | |2 WOS |a WOS:000285149100002 |
| 024 | 7 | _ | |2 ISSN |a 1477-2639 |
| 037 | _ | _ | |a PreJuSER-15171 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 540 |
| 084 | _ | _ | |2 WoS |a Chemistry, Inorganic & Nuclear |
| 084 | _ | _ | |2 WoS |a Environmental Sciences |
| 100 | 1 | _ | |0 P:(DE-HGF)0 |a Meyer, A. |b 0 |
| 245 | _ | _ | |a A new 15N tracer method to determine N turnover and denitrification of Pseudomonas stutzeri |
| 260 | _ | _ | |a London [u.a.] |b Taylor and Francis |c 2010 |
| 300 | _ | _ | |a 409 - 421 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |0 2918 |a Isotopes in Environmental and Health Studies |v 46 |x 1025-6016 |y 4 |
| 500 | _ | _ | |a This work was funded by the Deutsche Forschungsgemeinschaft (DFG) within the Beech Research Group (FOR 788), the EU-funded integrated project NitroEurope and by the Joint Helmholtz/Chinese Academy of Sciences Laboratory ENTRANCE. |
| 520 | _ | _ | |a Although denitrification is one of the key processes of ecosystem N turnover, the understanding of the regulation of the denitrification pathway is still limited due to the lack of feasible methods for the quantification of N₂ formation. Based on the previously developed isotope pairing method, we present a new in vitro ¹⁵N tracer method for the quantification of N₂ released from denitrification by bacterial cultures. The application of the new method was enabled by replacing the background air in the sample flasks with a gas mixture of He and O₂ with an approximately 50-fold reduced N₂ background (1.7% v/v), allowing for a direct and sensitive quantification of N₂ formation with isotope-ratio mass spectrometry after ¹⁵N-labelling on the one hand, but leaving the method relatively insensitive to intrusion of ambient N₂ on the other hand. The method was tested on bacterial cultures of Pseudomonas stutzeri grown at different oxygen levels. Additionally, NO and N₂O formation were determined with a chemoluminescence analyser and a gas chromatograph, respectively. Following labelling with ¹⁵N-ammonium and ¹⁵N-nitrate, it could be shown that P. stutzeri used ammonium preferably for biomass build-up, and nitrate preferably as electron acceptor. Between 84-107% of the total available N could be recovered. Due to the high sensitivity of the new method only low levels of ¹⁵N tracer were necessary, minimising substrate-induced effects and making this method also an appropriate tool for the use on soil cores. By that it offers a new method for studying denitrification in terrestrial ecosystems. |
| 536 | _ | _ | |0 G:(DE-Juel1)FUEK407 |2 G:(DE-HGF) |a Terrestrische Umwelt |c P24 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Denitrification |
| 650 | _ | 2 | |2 MeSH |a Nitrogen Isotopes: metabolism |
| 650 | _ | 2 | |2 MeSH |a Pseudomonas stutzeri: metabolism |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Nitrogen Isotopes |
| 650 | _ | 7 | |2 WoSType |a J |
| 653 | 2 | 0 | |2 Author |a denitrification |
| 653 | 2 | 0 | |2 Author |a isotope pairing |
| 653 | 2 | 0 | |2 Author |a Pseudomonas stutzeri |
| 653 | 2 | 0 | |2 Author |a nitrogen-15 |
| 653 | 2 | 0 | |2 Author |a isotope ecology |
| 653 | 2 | 0 | |2 Author |a tracer technique |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Bergmann, J. |b 1 |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Butterbach-Bahl, K. |b 2 |
| 700 | 1 | _ | |0 P:(DE-Juel1)142357 |a Brüggemann, N. |b 3 |u FZJ |
| 773 | _ | _ | |0 PERI:(DE-600)2100190-X |a 10.1080/10256016.2010.528840 |g Vol. 46, p. 409 - 421 |p 409 - 421 |q 46<409 - 421 |t Isotopes in Environmental and Health Studies |v 46 |x 1025-6016 |y 2010 |
| 856 | 7 | _ | |u http://dx.doi.org/10.1080/10256016.2010.528840 |
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| 914 | 1 | _ | |a Nachtrag |y 2010 |
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