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000015172 084__ $$2WoS$$aBiology
000015172 1001_ $$0P:(DE-HGF)0$$aGhirardo, A.$$b0
000015172 245__ $$aBiogenic Volatile Organic Compound and Respiratory CO2 Emissions after 13C-Labeling: Online Tracing of C Translocation Dynamics in Poplar Plants
000015172 260__ $$aLawrence, Kan.$$bPLoS$$c2011
000015172 300__ $$ae17393
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000015172 440_0 $$018181$$aPLOS One$$v6$$x1932-6203$$y2
000015172 500__ $$3POF3_Assignment on 2016-02-29
000015172 500__ $$aFinancial support was given by the Human Frontier Science Program ( to J. P. S. and A. G.) and the German Research Foundation ( to J. P. S., DFG SCHN653/4). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
000015172 520__ $$aGlobally plants are the primary sink of atmospheric CO(2), but are also the major contributor of a large spectrum of atmospheric reactive hydrocarbons such as terpenes (e.g. isoprene) and other biogenic volatile organic compounds (BVOC). The prediction of plant carbon (C) uptake and atmospheric oxidation capacity are crucial to define the trajectory and consequences of global environmental changes. To achieve this, the biosynthesis of BVOC and the dynamics of C allocation and translocation in both plants and ecosystems are important.We combined tunable diode laser absorption spectrometry (TDLAS) and proton transfer reaction mass spectrometry (PTR-MS) for studying isoprene biosynthesis and following C fluxes within grey poplar (Populus x canescens) saplings. This was achieved by feeding either (13)CO(2) to leaves or (13)C-glucose to shoots via xylem uptake. The translocation of (13)CO(2) from the source to other plant parts could be traced by (13)C-labeled isoprene and respiratory (13)CO(2) emission.In intact plants, assimilated (13)CO(2) was rapidly translocated via the phloem to the roots within 1 hour, with an average phloem transport velocity of 20.3±2.5 cm h(-1). (13)C label was stored in the roots and partially reallocated to the plants' apical part one day after labeling, particularly in the absence of photosynthesis. The daily C loss as BVOC ranged between 1.6% in mature leaves and 7.0% in young leaves. Non-isoprene BVOC accounted under light conditions for half of the BVOC C loss in young leaves and one-third in mature leaves. The C loss as isoprene originated mainly (76-78%) from recently fixed CO(2), to a minor extent from xylem-transported sugars (7-11%) and from photosynthetic intermediates with slower turnover rates (8-11%).We quantified the plants' C loss as respiratory CO(2) and BVOC emissions, allowing in tandem with metabolic analysis to deepen our understanding of ecosystem C flux.
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000015172 650_2 $$2MeSH$$aAlgorithms
000015172 650_2 $$2MeSH$$aBiological Transport: physiology
000015172 650_2 $$2MeSH$$aCarbon: pharmacokinetics
000015172 650_2 $$2MeSH$$aCarbon Dioxide: metabolism
000015172 650_2 $$2MeSH$$aCarbon Isotopes: pharmacokinetics
000015172 650_2 $$2MeSH$$aCell Respiration: physiology
000015172 650_2 $$2MeSH$$aModels, Biological
000015172 650_2 $$2MeSH$$aOnline Systems: instrumentation
000015172 650_2 $$2MeSH$$aPlant Leaves: metabolism
000015172 650_2 $$2MeSH$$aPlant Shoots: metabolism
000015172 650_2 $$2MeSH$$aPlants: metabolism
000015172 650_2 $$2MeSH$$aPopulus: metabolism
000015172 650_2 $$2MeSH$$aStaining and Labeling: instrumentation
000015172 650_2 $$2MeSH$$aStaining and Labeling: methods
000015172 650_2 $$2MeSH$$aVolatile Organic Compounds: metabolism
000015172 650_7 $$00$$2NLM Chemicals$$aCarbon Isotopes
000015172 650_7 $$00$$2NLM Chemicals$$aVolatile Organic Compounds
000015172 650_7 $$0124-38-9$$2NLM Chemicals$$aCarbon Dioxide
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000015172 7001_ $$0P:(DE-HGF)0$$aGutknecht, J.$$b1
000015172 7001_ $$0P:(DE-HGF)0$$aZimmer, I.$$b2
000015172 7001_ $$0P:(DE-Juel1)142357$$aBrüggemann, N.$$b3$$uFZJ
000015172 7001_ $$0P:(DE-HGF)0$$aSchnitzler, J.P.$$b4
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000015172 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3046154
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