TY  - JOUR
AU  - Ghirardo, A.
AU  - Gutknecht, J.
AU  - Zimmer, I.
AU  - Brüggemann, N.
AU  - Schnitzler, J.P.
TI  - Biogenic Volatile Organic Compound and Respiratory CO2 Emissions after 13C-Labeling: Online Tracing of C Translocation Dynamics in Poplar Plants
JO  - PLoS one
VL  - 6
SN  - 1932-6203
CY  - Lawrence, Kan.
PB  - PLoS
M1  - PreJuSER-15172
SP  - e17393
PY  - 2011
N1  - Financial support was given by the Human Frontier Science Program ( to J. P. S. and A. G.) and the German Research Foundation ( to J. P. S., DFG SCHN653/4). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
AB  - Globally plants are the primary sink of atmospheric CO(2), but are also the major contributor of a large spectrum of atmospheric reactive hydrocarbons such as terpenes (e.g. isoprene) and other biogenic volatile organic compounds (BVOC). The prediction of plant carbon (C) uptake and atmospheric oxidation capacity are crucial to define the trajectory and consequences of global environmental changes. To achieve this, the biosynthesis of BVOC and the dynamics of C allocation and translocation in both plants and ecosystems are important.We combined tunable diode laser absorption spectrometry (TDLAS) and proton transfer reaction mass spectrometry (PTR-MS) for studying isoprene biosynthesis and following C fluxes within grey poplar (Populus x canescens) saplings. This was achieved by feeding either (13)CO(2) to leaves or (13)C-glucose to shoots via xylem uptake. The translocation of (13)CO(2) from the source to other plant parts could be traced by (13)C-labeled isoprene and respiratory (13)CO(2) emission.In intact plants, assimilated (13)CO(2) was rapidly translocated via the phloem to the roots within 1 hour, with an average phloem transport velocity of 20.3±2.5 cm h(-1). (13)C label was stored in the roots and partially reallocated to the plants' apical part one day after labeling, particularly in the absence of photosynthesis. The daily C loss as BVOC ranged between 1.6% in mature leaves and 7.0% in young leaves. Non-isoprene BVOC accounted under light conditions for half of the BVOC C loss in young leaves and one-third in mature leaves. The C loss as isoprene originated mainly (76-78%) from recently fixed CO(2), to a minor extent from xylem-transported sugars (7-11%) and from photosynthetic intermediates with slower turnover rates (8-11%).We quantified the plants' C loss as respiratory CO(2) and BVOC emissions, allowing in tandem with metabolic analysis to deepen our understanding of ecosystem C flux.
KW  - Algorithms
KW  - Biological Transport: physiology
KW  - Carbon: pharmacokinetics
KW  - Carbon Dioxide: metabolism
KW  - Carbon Isotopes: pharmacokinetics
KW  - Cell Respiration: physiology
KW  - Models, Biological
KW  - Online Systems: instrumentation
KW  - Plant Leaves: metabolism
KW  - Plant Shoots: metabolism
KW  - Plants: metabolism
KW  - Populus: metabolism
KW  - Staining and Labeling: instrumentation
KW  - Staining and Labeling: methods
KW  - Volatile Organic Compounds: metabolism
KW  - Carbon Isotopes (NLM Chemicals)
KW  - Volatile Organic Compounds (NLM Chemicals)
KW  - Carbon Dioxide (NLM Chemicals)
KW  - Carbon (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21387007
C2  - pmc:PMC3046154
UR  - <Go to ISI:>//WOS:000287931400068
DO  - DOI:10.1371/journal.pone.0017393
UR  - https://juser.fz-juelich.de/record/15172
ER  -