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@ARTICLE{Ghirardo:15172,
      author       = {Ghirardo, A. and Gutknecht, J. and Zimmer, I. and
                      Brüggemann, N. and Schnitzler, J.P.},
      title        = {{B}iogenic {V}olatile {O}rganic {C}ompound and
                      {R}espiratory {CO}2 {E}missions after 13{C}-{L}abeling:
                      {O}nline {T}racing of {C} {T}ranslocation {D}ynamics in
                      {P}oplar {P}lants},
      journal      = {PLoS one},
      volume       = {6},
      issn         = {1932-6203},
      address      = {Lawrence, Kan.},
      publisher    = {PLoS},
      reportid     = {PreJuSER-15172},
      pages        = {e17393},
      year         = {2011},
      note         = {Financial support was given by the Human Frontier Science
                      Program ( to J. P. S. and A. G.) and the German Research
                      Foundation ( to J. P. S., DFG SCHN653/4). The funders had no
                      role in study design, data collection and analysis, decision
                      to publish, or preparation of the manuscript.},
      abstract     = {Globally plants are the primary sink of atmospheric CO(2),
                      but are also the major contributor of a large spectrum of
                      atmospheric reactive hydrocarbons such as terpenes (e.g.
                      isoprene) and other biogenic volatile organic compounds
                      (BVOC). The prediction of plant carbon (C) uptake and
                      atmospheric oxidation capacity are crucial to define the
                      trajectory and consequences of global environmental changes.
                      To achieve this, the biosynthesis of BVOC and the dynamics
                      of C allocation and translocation in both plants and
                      ecosystems are important.We combined tunable diode laser
                      absorption spectrometry (TDLAS) and proton transfer reaction
                      mass spectrometry (PTR-MS) for studying isoprene
                      biosynthesis and following C fluxes within grey poplar
                      (Populus x canescens) saplings. This was achieved by feeding
                      either (13)CO(2) to leaves or (13)C-glucose to shoots via
                      xylem uptake. The translocation of (13)CO(2) from the source
                      to other plant parts could be traced by (13)C-labeled
                      isoprene and respiratory (13)CO(2) emission.In intact
                      plants, assimilated (13)CO(2) was rapidly translocated via
                      the phloem to the roots within 1 hour, with an average
                      phloem transport velocity of 20.3±2.5 cm h(-1). (13)C label
                      was stored in the roots and partially reallocated to the
                      plants' apical part one day after labeling, particularly in
                      the absence of photosynthesis. The daily C loss as BVOC
                      ranged between $1.6\%$ in mature leaves and $7.0\%$ in young
                      leaves. Non-isoprene BVOC accounted under light conditions
                      for half of the BVOC C loss in young leaves and one-third in
                      mature leaves. The C loss as isoprene originated mainly
                      $(76-78\%)$ from recently fixed CO(2), to a minor extent
                      from xylem-transported sugars $(7-11\%)$ and from
                      photosynthetic intermediates with slower turnover rates
                      $(8-11\%).We$ quantified the plants' C loss as respiratory
                      CO(2) and BVOC emissions, allowing in tandem with metabolic
                      analysis to deepen our understanding of ecosystem C flux.},
      keywords     = {Algorithms / Biological Transport: physiology / Carbon:
                      pharmacokinetics / Carbon Dioxide: metabolism / Carbon
                      Isotopes: pharmacokinetics / Cell Respiration: physiology /
                      Models, Biological / Online Systems: instrumentation / Plant
                      Leaves: metabolism / Plant Shoots: metabolism / Plants:
                      metabolism / Populus: metabolism / Staining and Labeling:
                      instrumentation / Staining and Labeling: methods / Volatile
                      Organic Compounds: metabolism / Carbon Isotopes (NLM
                      Chemicals) / Volatile Organic Compounds (NLM Chemicals) /
                      Carbon Dioxide (NLM Chemicals) / Carbon (NLM Chemicals) / J
                      (WoSType)},
      cin          = {IBG-3},
      ddc          = {500},
      cid          = {I:(DE-Juel1)IBG-3-20101118},
      pnm          = {Terrestrische Umwelt},
      pid          = {G:(DE-Juel1)FUEK407},
      shelfmark    = {Biology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:21387007},
      pmc          = {pmc:PMC3046154},
      UT           = {WOS:000287931400068},
      doi          = {10.1371/journal.pone.0017393},
      url          = {https://juser.fz-juelich.de/record/15172},
}