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000151777 1001_ $$0P:(DE-HGF)0$$aShvetsov, Alexey V.$$b0$$eCorresponding author
000151777 245__ $$aStructure of RecX protein complex with the presynaptic RecA filament: Molecular dynamics simulations and small angle neutron scattering
000151777 260__ $$aAmsterdam [u.a.]$$bElsevier$$c2014
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000151777 520__ $$aUsing molecular modeling techniques we have built the full atomic structure and performed molecular dynamics simulations for the complexes formed by Escherichia coli RecX protein with a single-stranded oligonucleotide and with RecA presynaptic filament. Based on the modeling and SANS experimental data a sandwich-like filament structure formed two chains of RecX monomers bound to the opposite sides of the single stranded DNA is proposed for RecX::ssDNA complex. The model for RecX::RecA::ssDNA include RecX binding into the grove of RecA::ssDNA filament that occurs mainly via Coulomb interactions between RecX and ssDNA. Formation of RecX::RecA::ssDNA filaments in solution was confirmed by SANS measurements which were in agreement with the spectra computed from the molecular dynamics simulations.
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000151777 7001_ $$0P:(DE-HGF)0$$aLebedev, Dmitry V.$$b1
000151777 7001_ $$0P:(DE-HGF)0$$aChervyakova, Daria B.$$b2
000151777 7001_ $$0P:(DE-HGF)0$$aBakhlanova, Irina V.$$b3
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000151777 7001_ $$0P:(DE-HGF)0$$aKuklin, Aleksandr I.$$b6
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000151777 7001_ $$0P:(DE-HGF)0$$aIsaev-Ivanov, Vladimir V.$$b8
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