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@ARTICLE{Shvetsov:151777,
author = {Shvetsov, Alexey V. and Lebedev, Dmitry V. and Chervyakova,
Daria B. and Bakhlanova, Irina V. and Yung, Igor A. and
Radulescu, Aurel and Kuklin, Aleksandr I. and Baitin, Dmitry
M. and Isaev-Ivanov, Vladimir V.},
title = {{S}tructure of {R}ec{X} protein complex with the
presynaptic {R}ec{A} filament: {M}olecular dynamics
simulations and small angle neutron scattering},
journal = {FEBS letters},
volume = {588},
number = {6},
issn = {0014-5793},
address = {Amsterdam [u.a.]},
publisher = {Elsevier},
reportid = {FZJ-2014-01659},
pages = {948–955},
year = {2014},
abstract = {Using molecular modeling techniques we have built the full
atomic structure and performed molecular dynamics
simulations for the complexes formed by Escherichia coli
RecX protein with a single-stranded oligonucleotide and with
RecA presynaptic filament. Based on the modeling and SANS
experimental data a sandwich-like filament structure formed
two chains of RecX monomers bound to the opposite sides of
the single stranded DNA is proposed for RecX::ssDNA complex.
The model for RecX::RecA::ssDNA include RecX binding into
the grove of RecA::ssDNA filament that occurs mainly via
Coulomb interactions between RecX and ssDNA. Formation of
RecX::RecA::ssDNA filaments in solution was confirmed by
SANS measurements which were in agreement with the spectra
computed from the molecular dynamics simulations.},
cin = {Neutronenstreuung ; JCNS-1 / JCNS (München) ; Jülich
Centre for Neutron Science JCNS (München) ; JCNS-FRM-II /
ICS-1},
ddc = {570},
cid = {I:(DE-Juel1)JCNS-1-20110106 /
I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)ICS-1-20110106},
pnm = {451 - Soft Matter Composites (POF2-451) / 54G - JCNS
(POF2-54G24)},
pid = {G:(DE-HGF)POF2-451 / G:(DE-HGF)POF2-54G24},
experiment = {EXP:(DE-MLZ)KWS2-20140101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000333088800016},
doi = {10.1016/j.febslet.2014.01.053},
url = {https://juser.fz-juelich.de/record/151777},
}