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@ARTICLE{Lu:153359,
author = {Lu, Alvin and Magupalli, Venkat Giri and Ruan, Jianbin and
Yin, Qian and Atianand, Maninjay K. and Vos, Matthijn R. and
Schröder, Gunnar F. and Fitzgerald, Katherine A. and Wu,
Hao and Egelman, Edward H.},
title = {{U}nified {P}olymerization {M}echanism for the {A}ssembly
of {ASC}-{D}ependent {I}nflammasomes},
journal = {Cell},
volume = {156},
number = {6},
issn = {0092-8674},
address = {[Cambridge, Mass.]},
publisher = {Cell Press},
reportid = {FZJ-2014-02990},
pages = {1193 - 1206},
year = {2014},
note = {Bitte ändern Sie "Schröder, Gunnar" in "Schröder, Gunnar
F." um.},
abstract = {Inflammasomes elicit host defense inside cells by
activating caspase-1 for cytokine maturation and cell death.
AIM2 and NLRP3 are representative sensor proteins in two
major families of inflammasomes. The adaptor protein ASC
bridges the sensor proteins and caspase-1 to form ternary
inflammasome complexes, achieved through pyrin domain (PYD)
interactions between sensors and ASC and through caspase
activation and recruitment domain (CARD) interactions
between ASC and caspase-1. We found that PYD and CARD both
form filaments. Activated AIM2 and NLRP3 nucleate PYD
filaments of ASC, which, in turn, cluster the CARD of ASC.
ASC thus nucleates CARD filaments of caspase-1, leading to
proximity-induced activation. Endogenous NLRP3 inflammasome
is also filamentous. The cryoelectron microscopy structure
of ASCPYD filament at near-atomic resolution provides a
template for homo- and hetero-PYD/PYD associations, as
confirmed by structure-guided mutagenesis. We propose that
ASC-dependent inflammasomes in both families share a unified
assembly mechanism that involves two successive steps of
nucleation-induced polymerization.},
cin = {ICS-6},
ddc = {570},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {452 - Structural Biology (POF2-452)},
pid = {G:(DE-HGF)POF2-452},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000332945100010},
pubmed = {pmid:24630722},
doi = {10.1016/j.cell.2014.02.008},
url = {https://juser.fz-juelich.de/record/153359},
}