TY  - JOUR
AU  - Klein, Rebecca
AU  - Blaschke, Stefan
AU  - Neumaier, Bernd
AU  - Endepols, Heike
AU  - Graf, Rudolf
AU  - Keuters, Meike
AU  - Hucklenbroich, Joerg
AU  - Albrechtsen, Morten
AU  - Rees, Stephen
AU  - Fink, Gereon Rudolf
AU  - Schroeter, Michael
AU  - Rueger, Maria Adele
TI  - The synthetic NCAM mimetic peptide FGL mobilizes neural stem cells in vitro and in vivo
JO  - Stem cell reviews and reports
VL  - 10
IS  - 4
SN  - 1558-6804
CY  - New York, NY
PB  - Springer
M1  - FZJ-2014-03158
SP  - 539-547 
PY  - 2014
AB  - The neural cell adhesion molecule (NCAM) plays a role in neurite outgrowth, synaptogenesis, and neuronal differentiation. The NCAM mimetic peptide FG Loop (FGL) promotes neuronal survival in vitro and enhances spatial learning and memory in rats. We here investigated the effects of FGL on neural stem cells (NSC) in vitro and in vivo. In vitro, cell proliferation of primary NSC was assessed after exposure to various concentrations of NCAM or FGL. The differentiation potential of NCAM- or FGL-treated cells was assessed immunocytochemically. To investigate its influence on endogenous NSC in vivo, FGL was injected subcutaneously into adult rats. The effects on NSC mobilization were studied both via non-invasive positron emission tomography (PET) imaging using the tracer [18F]-fluoro-l-thymidine ([18F]FLT), as well as with immunohistochemistry. Only FGL significantly enhanced NSC proliferation in vitro, with a maximal effect at 10 μg/ml. During differentiation, NCAM promoted neurogenesis, while FGL induced an oligodendroglial phenotype; astrocytic differentiation was neither affected by NCAM or FGL. Those differential effects of NCAM and FGL on differentiation were mediated through different receptors. After FGL-injection in vivo, proliferative activity of NSC in the subventricular zone (SVZ) was increased (compared to placebo-treated animals). Moreover, non-invasive imaging of cell proliferation using [18F]FLT-PET supported an FGL-induced mobilization of NSC from both the SVZ and the hippocampus. We conclude that FGL robustly induces NSC mobilization in vitro and in vivo, and supports oligodendroglial differentiation. This capacity renders FGL a promising agent to facilitate remyelinization, which may eventually make FGL a drug candidate for demyelinating neurological disorders
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000340510300008
C6  - pmid:24817672
DO  - DOI:10.1007/s12015-014-9512-5
UR  - https://juser.fz-juelich.de/record/153654
ER  -