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@ARTICLE{Ghirardo:154079,
      author       = {Ghirardo, A. and Wright, L. P. and Bi, Z. and Rosenkranz,
                      M. and Pulido, P. and Rodriguez-Concepcion, M. and
                      Niinemets, U. and Bruggemann, N. and Gershenzon, J. and
                      Schnitzler, J.-P.},
      title        = {{M}etabolic {F}lux {A}nalysis of {P}lastidic {I}soprenoid
                      {B}iosynthesis in {P}oplar {L}eaves {E}mitting and
                      {N}onemitting {I}soprene},
      journal      = {Plant physiology},
      volume       = {165},
      number       = {1},
      issn         = {1532-2548},
      address      = {Rockville, Md.: Soc.},
      publisher    = {JSTOR},
      reportid     = {FZJ-2014-03481},
      pages        = {37 - 51},
      year         = {2014},
      abstract     = {The plastidic 2-C-methyl-D-erythritol-4-phosphate (MEP)
                      pathway is one of the most important pathways in plants
                      andproduces a large variety of essential isoprenoids. Its
                      regulation, however, is still not well understood. Using the
                      stableisotope 13C-labeling technique, we analyzed the carbon
                      fluxes through the MEP pathway and into the major
                      plastidicisoprenoid products in isoprene-emitting and
                      transgenic isoprene-nonemitting (NE) gray poplar (Populus 3
                      canescens). Weassessed the dependence on temperature, light
                      intensity, and atmospheric [CO2]. Isoprene biosynthesis was
                      by far $(99\%)$ themain carbon sink of MEP pathway
                      intermediates in mature gray poplar leaves, and its
                      production required severalfold highercarbon fluxes compared
                      with NE leaves with almost zero isoprene emission. To
                      compensate for the much lower demand forcarbon, NE leaves
                      drastically reduced the overall carbon flux within the MEP
                      pathway. Feedback inhibition of
                      1-deoxy-Dxylulose-5-phosphate synthase activity by
                      accumulated plastidic dimethylallyl diphosphate almost
                      completely explained thisreduction in carbon flux. Our data
                      demonstrate that short-term biochemical feedback regulation
                      of 1-deoxy-D-xylulose-5-phosphate synthase activity by
                      plastidic dimethylallyl diphosphate is an important
                      regulatory mechanism of the MEPpathway. Despite being
                      relieved from the large carbon demand of isoprene
                      biosynthesis, NE plants redirected onlyapproximately $0.5\%$
                      of this saved carbon toward essential nonvolatile
                      isoprenoids, i.e. b-carotene and lutein, most probablyto
                      compensate for the absence of isoprene and its antioxidant
                      properties.},
      cin          = {IBG-3},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-3-20101118},
      pnm          = {246 - Modelling and Monitoring Terrestrial Systems: Methods
                      and Technologies (POF2-246) / 255 - Terrestrial Systems:
                      From Observation to Prediction (POF3-255)},
      pid          = {G:(DE-HGF)POF2-246 / G:(DE-HGF)POF3-255},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000335906300004},
      pubmed       = {pmid:24590857},
      doi          = {10.1104/pp.114.236018},
      url          = {https://juser.fz-juelich.de/record/154079},
}