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@ARTICLE{Schmitz:154411,
      author       = {Schmitz, Sabine and Pinkawa, Michael and Eble, Michael J.
                      and Kriehuber, Ralf},
      title        = {{P}ersisting ring chromosomes detected by m{FISH} in
                      lymphocytes of a cancer patient—{A} case report},
      journal      = {Mutation research / Genetic toxicology and environmental
                      mutagenesis},
      volume       = {756},
      number       = {1-2},
      issn         = {1383-5718},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2014-03758},
      pages        = {158 - 164},
      year         = {2013},
      abstract     = {We report the case of an 84 years old prostate cancer
                      patient with severe side effects after radiotherapy in 2006.
                      He was cytogenetically analysed in 2009 and in 2012 in a
                      comparative study for individual radiosensitivity of
                      prostate cancer patients. No other patient had clonal
                      aberrations, but this patient showed ring chromosomes in the
                      range of $21–25\%$ of lymphocytes. He received 5 cycles of
                      5-fluorouracil/folic acid for chemotherapy of sigmoid colon
                      carcinoma in 2003, three years before radiotherapy of
                      prostate cancer.Blood samples were irradiated ex vivo with
                      Cs-137 γ-rays (0.7 Gy/min) in the G0-phase of the cell
                      cycle. 100 FISH painted metaphases were analysed for the
                      control and the irradiated samples each. Multicolour in situ
                      hybridisation techniques like mFISH and mBand as well as MYC
                      locus, telomere and centromere painting probes were used to
                      characterise ring metaphases. Metaphase search and
                      autocapture was performed with a Zeiss Axioplan 2 imaging
                      microscope followed by scoring and image analysis using
                      Metafer 4/ISIS software (MetaSystems).In 2009 chromosome 8
                      rings were found in about $25\%$ of lymphocytes. Rings were
                      stable over time and increased to about $30\%$ until 2012.
                      The ring chromosome 8 always lacked telomere signals and a
                      small amount of rings displayed up to four centromere
                      signals. In aberrant metaphases 8pter and 8qter were either
                      translocated or deleted. Further analyses revealed that the
                      breakpoint at the p arm is localised at 8p21.2–22. The
                      breakpoint at the q arm turned out to be distal from the MYC
                      locus at 8q23–24.We hypothesise that the ring chromosome 8
                      has been developed during the 5 FU/folic acid treatments in
                      2003. The long term persistence might be due to clonal
                      expansion of a damaged but viable hematopoietic stem cell
                      giving rise to cycling progenitor cells that permit cell
                      survival and proliferation.},
      cin          = {S-US},
      ddc          = {570},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF2-899)},
      pid          = {G:(DE-HGF)POF2-899},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000325600300022},
      doi          = {10.1016/j.mrgentox.2013.06.008},
      url          = {https://juser.fz-juelich.de/record/154411},
}