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@INPROCEEDINGS{Brzozowska:154627,
      author       = {Brzozowska, Kinga and Pomplun, Ekkehard and Kriehuber,
                      Ralf},
      title        = {{C}hromosome aberrations in human peripheral blood
                      lymphocytes and h{TERT}-{RPE}1 cell line after exposure to
                      the {A}uger electron emitter {I}-125},
      reportid     = {FZJ-2014-03917},
      pages        = {44},
      year         = {2011},
      abstract     = {Introduction: It has been shown that I-123-labeled estrogen
                      treatment induced chromosome aberrations with an efficiency
                      of about one aberration for each 1000 disintegrations in
                      estrogen receptor-positive Chinese hamster ovary (CHO-ER)
                      cells. RBE values for chromatid damage induced by Zn-65
                      compared to γ-radiation were found to be in the range from
                      about 1 - 3 repectively 2 - 5 depending on the assumed
                      intracellular distribution pattern of Zn-65. To elucidate
                      the genotoxic potential of DNA-associated AEE, chromosomal
                      /chromatid aberrations were analyzed in
                      Iodine-125-deoxyuridine (I-125-UdR) exposed human peripheral
                      blood lymphocytes (PBL) and in human telomerase-immortalized
                      retinal pigment epithelial cells (hTERT-RPE1).Methods: For
                      each donor, PBL were incubated with I-125-UdR for 5 h (2.5
                      and 5 kBq/ml). The culture was fixed for aberrations at 72 h
                      post-stimulation. hTERT-RPE1 cells were exposed to I-125-UdR
                      activities ranging from 0.5 – 4 kBq/ml. The cells were
                      harvested for aberrations at 26 h after initiation of the
                      culture. All slides were stained with 10 $\%$ Giemsa. For
                      each dose point 100 metaphases were analysed. For both PBL
                      and hTERT-RPE1 cells, cell cycle analysis using EdU Flow
                      Cytometry Assay Kits (Invitrogen) was performed.Results: Our
                      preliminary data show that I-125-UdR primarily induce
                      chromatid-type aberrations. An enhanced level of aberrations
                      was observed at already 100 accumulated decays per cell,
                      whereof one decay per cell per 20 min was calculated. The
                      cell cycle of both PBL and hTERT-RPE1 cell line are delayed
                      due to incorporation of I-125-UdR in the DNA, when compared
                      to control cells. Conclusions: I-125-UdR posses a strong
                      genotoxic capacity in PBL and hTERT-RPE1 cells even at very
                      low numbers of accumulated decays per cell and hence at a
                      very low dose rate (< 11 mGy/hour).Funded by
                      Bundesministerium für Bildung und Forschung (BMBF),
                      Kompetenzverbund Strahlenforschung (KVSF), Project No.:
                      02NUK005A},
      month         = {Aug},
      date          = {2011-08-24},
      organization  = {7th International Symposium on
                       Physical, Molecular, Cellular and
                       Medical Aspects of Auger Processes,
                       Jülich (Germany), 24 Aug 2011 - 26 Aug
                       2011},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF2-899)},
      pid          = {G:(DE-HGF)POF2-899},
      typ          = {PUB:(DE-HGF)8},
      url          = {https://juser.fz-juelich.de/record/154627},
}