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000154750 1001_ $$0P:(DE-HGF)0$$aHong, Liang$$b0$$eCorresponding Author
000154750 245__ $$aStructure and Dynamics of a Compact State of a Multidomain Protein, the Mercuric Ion Reductase
000154750 260__ $$aNew York, NY$$bRockefeller Univ. Press$$c2014
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000154750 520__ $$aThe functional efficacy of colocalized, linked protein domains is dependent on linker flexibility and system compaction. However, the detailed characterization of these properties in aqueous solution presents an enduring challenge. Here, we employ a novel, to our knowledge, combination of complementary techniques, including small-angle neutron scattering, neutron spin-echo spectroscopy, and all-atom molecular dynamics and coarse-grained simulation, to identify and characterize in detail the structure and dynamics of a compact form of mercuric ion reductase (MerA), an enzyme central to bacterial mercury resistance. MerA possesses metallochaperone-like N-terminal domains (NmerA) tethered to its catalytic core domain by linkers. The NmerA domains are found to interact principally through electrostatic interactions with the core, leashed by the linkers so as to subdiffuse on the surface over an area close to the core C-terminal Hg(II)-binding cysteines. How this compact, dynamical arrangement may facilitate delivery of Hg(II) from NmerA to the core domain is discussed.
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000154750 65017 $$0V:(DE-MLZ)GC-140$$2V:(DE-HGF)$$aSoft Matter, Macromolecules, Complex fluids, Biophysics$$x0
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000154750 7001_ $$0P:(DE-HGF)0$$aSharp, Melissa&nbsp$$b1
000154750 7001_ $$0P:(DE-Juel1)144349$$aPoblete, Simón$$b2
000154750 7001_ $$0P:(DE-Juel1)130542$$aBiehl, Ralf$$b3
000154750 7001_ $$0P:(DE-Juel1)131056$$aZamponi, Michaela$$b4
000154750 7001_ $$0P:(DE-Juel1)145431$$aSzekely, Noemi$$b5
000154750 7001_ $$0P:(DE-Juel1)130507$$aAppavou, Marie-Sousai$$b6
000154750 7001_ $$0P:(DE-Juel1)131039$$aWinkler, Roland G.$$b7
000154750 7001_ $$0P:(DE-HGF)0$$aNauss, Rachel E.$$b8
000154750 7001_ $$0P:(DE-HGF)0$$aJohs, Alexander$$b9
000154750 7001_ $$0P:(DE-HGF)0$$aParks, Jerry&nbsp$$b10
000154750 7001_ $$0P:(DE-HGF)0$$aYi, Zheng$$b11
000154750 7001_ $$0P:(DE-HGF)0$$aCheng, Xiaolin$$b12
000154750 7001_ $$0P:(DE-HGF)0$$aLiang, Liyuan$$b13
000154750 7001_ $$0P:(DE-Juel1)130872$$aOhl, Michael$$b14
000154750 7001_ $$0P:(DE-HGF)0$$aMiller, Susan&nbsp$$b15
000154750 7001_ $$0P:(DE-Juel1)130917$$aRichter, Dieter$$b16
000154750 7001_ $$0P:(DE-Juel1)130665$$aGompper, Gerhard$$b17
000154750 7001_ $$0P:(DE-HGF)0$$aSmith, Jeremy&nbsp$$b18
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