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@ARTICLE{Wingen:154801,
      author       = {Wingen, Marcus and Potzkei, Janko and Endres, Stephan and
                      Casini, Giorgia and Rupprecht, Christian and Fahlke,
                      Christoph and Krauss, Ulrich and Jaeger, Karl-Erich and
                      Drepper, Thomas and Gensch, Thomas},
      title        = {{T}he photophysics of {LOV}-based fluorescent proteins –
                      new tools for cell biology},
      journal      = {Photochemical $\&$ photobiological sciences},
      volume       = {13},
      number       = {6},
      issn         = {1474-905X},
      address      = {Cambridge},
      publisher    = {Royal Society of Chemistry},
      reportid     = {FZJ-2014-04069},
      pages        = {875-83},
      year         = {2014},
      abstract     = {LOV-based fluorescent proteins (FPs) are an alternative
                      class of fluorescent reporters with unique properties which
                      complement the well-established proteins of the GFP family.
                      One of the most important features of LOV-based FPs is the
                      independence of molecular oxygen for the development of
                      their specific fluorescence. Furthermore, they are
                      characterized by small size and rapid signal development.
                      Over the last few years, a number of different bacterial and
                      plant LOV-based fluorescent proteins such as FbFP, iLOV and
                      miniSOG have been developed and optimized. In this report,
                      we comparatively have characterized the photophysical
                      properties of nine different LOV-based fluorescent proteins
                      including the excitation and emission maxima, the extinction
                      coefficient, the fluorescence quantum yield, the average
                      fluorescence lifetime and the photostability. The unified
                      characterization of the LOV-based FPs provides a useful
                      guide to apply them as in vivo tools for quantitative
                      analyses and biological imaging.},
      cin          = {IMET / ICS-4},
      ddc          = {620},
      cid          = {I:(DE-Juel1)IMET-20090612 / I:(DE-Juel1)ICS-4-20110106},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:24500379},
      UT           = {WOS:000336802900005},
      doi          = {10.1039/C3PP50414J},
      url          = {https://juser.fz-juelich.de/record/154801},
}