000154975 001__ 154975
000154975 005__ 20210129214003.0
000154975 0247_ $$2doi$$a10.1016/j.biomaterials.2014.05.027
000154975 0247_ $$2ISSN$$a0142-9612
000154975 0247_ $$2ISSN$$a1878-5905
000154975 0247_ $$2WOS$$aWOS:000339035000012
000154975 0247_ $$2altmetric$$aaltmetric:2425631
000154975 0247_ $$2pmid$$apmid:24889032
000154975 037__ $$aFZJ-2014-04182
000154975 082__ $$a570
000154975 1001_ $$0P:(DE-HGF)0$$aHeras-Bautista, Carlos O.$$b0$$eCorresponding Author
000154975 245__ $$aThe influence of physiological matrix conditions on permanent culture of induced pluripotent stem cell-derived cardiomyocytes
000154975 260__ $$aAmsterdam [u.a.]$$bElsevier Science$$c2014
000154975 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1407767291_30524
000154975 3367_ $$2DataCite$$aOutput Types/Journal article
000154975 3367_ $$00$$2EndNote$$aJournal Article
000154975 3367_ $$2BibTeX$$aARTICLE
000154975 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000154975 3367_ $$2DRIVER$$aarticle
000154975 520__ $$aCardiomyocytes (CMs) from induced pluripotent stem (iPS) cells mark an important achievement in the development of in vitro pharmacological, toxicological and developmental assays and in the establishment of protocols for cardiac cell replacement therapy. Using CMs generated from murine embryonic stem cells and iPS cells we found increased cell–matrix interaction and more matured embryoid body (EB) structures in iPS cell-derived EBs. However, neither suspension-culture in form of purified cardiac clusters nor adherence-culture on traditional cell culture plastic allowed for extended culture of CMs. CMs grown for five weeks on polystyrene exhibit signs of massive mechanical stress as indicated by α-smooth muscle actin expression and loss of sarcomere integrity. Hydrogels from polyacrylamide allow adapting of the matrix stiffness to that of cardiac tissue. We were able to eliminate the bottleneck of low cell adhesion using 2,5-Dioxopyrrolidin-1-yl-6-acrylamidohexanoate as a crosslinker to immobilize matrix proteins on the gels surface. Finally we present an easy method to generate polyacrylamide gels with a physiological Young's modulus of 55 kPa and defined surface ligand, facilitating the culture of murine and human iPS-CMs, removing excess mechanical stresses and reducing the risk of tissue culture artifacts exerted by stiff substrates.
000154975 536__ $$0G:(DE-HGF)POF2-453$$a453 - Physics of the Cell (POF2-453)$$cPOF2-453$$fPOF II$$x0
000154975 588__ $$aDataset connected to CrossRef, juser.fz-juelich.de
000154975 7001_ $$0P:(DE-HGF)0$$aKatsen-Globa, Alisa$$b1
000154975 7001_ $$0P:(DE-HGF)0$$aSchloerer, Nils E.$$b2
000154975 7001_ $$0P:(DE-Juel1)128807$$aDieluweit, Sabine$$b3$$ufzj
000154975 7001_ $$0P:(DE-HGF)0$$aAbd El Aziz, Osama M.$$b4
000154975 7001_ $$0P:(DE-HGF)0$$aPeinkofer, Gabriel$$b5
000154975 7001_ $$0P:(DE-HGF)0$$aAttia, Wael A.$$b6
000154975 7001_ $$0P:(DE-HGF)0$$aKhalil, Markus$$b7
000154975 7001_ $$0P:(DE-HGF)0$$aBrockmeier, Konrad$$b8
000154975 7001_ $$0P:(DE-HGF)0$$aHescheler, Jürgen$$b9
000154975 7001_ $$0P:(DE-HGF)0$$aPfannkuche, Kurt$$b10
000154975 773__ $$0PERI:(DE-600)2004010-6$$a10.1016/j.biomaterials.2014.05.027$$gVol. 35, no. 26, p. 7374 - 7385$$n26$$p7374 - 7385$$tBiomaterials$$v35$$x0142-9612$$y2014
000154975 8564_ $$uhttps://juser.fz-juelich.de/record/154975/files/FZJ-2014-04182.pdf$$yRestricted
000154975 909CO $$ooai:juser.fz-juelich.de:154975$$pVDB
000154975 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)128807$$aForschungszentrum Jülich GmbH$$b3$$kFZJ
000154975 9132_ $$0G:(DE-HGF)POF3-552$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$aDE-HGF$$bKey Technologies$$lBioSoft  Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vEngineering Cell Function$$x0
000154975 9131_ $$0G:(DE-HGF)POF2-453$$1G:(DE-HGF)POF2-450$$2G:(DE-HGF)POF2-400$$3G:(DE-HGF)POF2$$4G:(DE-HGF)POF$$aDE-HGF$$bSchlüsseltechnologien$$lBioSoft$$vPhysics of the Cell$$x0
000154975 9141_ $$y2014
000154975 915__ $$0StatID:(DE-HGF)0010$$2StatID$$aJCR/ISI refereed
000154975 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR
000154975 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000154975 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000154975 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000154975 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000154975 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000154975 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000154975 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000154975 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz
000154975 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences
000154975 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000154975 920__ $$lyes
000154975 9201_ $$0I:(DE-Juel1)ICS-7-20110106$$kICS-7$$lBiomechanik$$x0
000154975 980__ $$ajournal
000154975 980__ $$aVDB
000154975 980__ $$aI:(DE-Juel1)ICS-7-20110106
000154975 980__ $$aUNRESTRICTED
000154975 981__ $$aI:(DE-Juel1)IBI-2-20200312