TY  - JOUR
AU  - Heras-Bautista, Carlos O.
AU  - Katsen-Globa, Alisa
AU  - Schloerer, Nils E.
AU  - Dieluweit, Sabine
AU  - Abd El Aziz, Osama M.
AU  - Peinkofer, Gabriel
AU  - Attia, Wael A.
AU  - Khalil, Markus
AU  - Brockmeier, Konrad
AU  - Hescheler, Jürgen
AU  - Pfannkuche, Kurt
TI  - The influence of physiological matrix conditions on permanent culture of induced pluripotent stem cell-derived cardiomyocytes
JO  - Biomaterials
VL  - 35
IS  - 26
SN  - 0142-9612
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - FZJ-2014-04182
SP  - 7374 - 7385
PY  - 2014
AB  - Cardiomyocytes (CMs) from induced pluripotent stem (iPS) cells mark an important achievement in the development of in vitro pharmacological, toxicological and developmental assays and in the establishment of protocols for cardiac cell replacement therapy. Using CMs generated from murine embryonic stem cells and iPS cells we found increased cell–matrix interaction and more matured embryoid body (EB) structures in iPS cell-derived EBs. However, neither suspension-culture in form of purified cardiac clusters nor adherence-culture on traditional cell culture plastic allowed for extended culture of CMs. CMs grown for five weeks on polystyrene exhibit signs of massive mechanical stress as indicated by α-smooth muscle actin expression and loss of sarcomere integrity. Hydrogels from polyacrylamide allow adapting of the matrix stiffness to that of cardiac tissue. We were able to eliminate the bottleneck of low cell adhesion using 2,5-Dioxopyrrolidin-1-yl-6-acrylamidohexanoate as a crosslinker to immobilize matrix proteins on the gels surface. Finally we present an easy method to generate polyacrylamide gels with a physiological Young's modulus of 55 kPa and defined surface ligand, facilitating the culture of murine and human iPS-CMs, removing excess mechanical stresses and reducing the risk of tissue culture artifacts exerted by stiff substrates.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000339035000012
C6  - pmid:24889032
DO  - DOI:10.1016/j.biomaterials.2014.05.027
UR  - https://juser.fz-juelich.de/record/154975
ER  -