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@ARTICLE{HerasBautista:154975,
      author       = {Heras-Bautista, Carlos O. and Katsen-Globa, Alisa and
                      Schloerer, Nils E. and Dieluweit, Sabine and Abd El Aziz,
                      Osama M. and Peinkofer, Gabriel and Attia, Wael A. and
                      Khalil, Markus and Brockmeier, Konrad and Hescheler, Jürgen
                      and Pfannkuche, Kurt},
      title        = {{T}he influence of physiological matrix conditions on
                      permanent culture of induced pluripotent stem cell-derived
                      cardiomyocytes},
      journal      = {Biomaterials},
      volume       = {35},
      number       = {26},
      issn         = {0142-9612},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2014-04182},
      pages        = {7374 - 7385},
      year         = {2014},
      abstract     = {Cardiomyocytes (CMs) from induced pluripotent stem (iPS)
                      cells mark an important achievement in the development of in
                      vitro pharmacological, toxicological and developmental
                      assays and in the establishment of protocols for cardiac
                      cell replacement therapy. Using CMs generated from murine
                      embryonic stem cells and iPS cells we found increased
                      cell–matrix interaction and more matured embryoid body
                      (EB) structures in iPS cell-derived EBs. However, neither
                      suspension-culture in form of purified cardiac clusters nor
                      adherence-culture on traditional cell culture plastic
                      allowed for extended culture of CMs. CMs grown for five
                      weeks on polystyrene exhibit signs of massive mechanical
                      stress as indicated by α-smooth muscle actin expression and
                      loss of sarcomere integrity. Hydrogels from polyacrylamide
                      allow adapting of the matrix stiffness to that of cardiac
                      tissue. We were able to eliminate the bottleneck of low cell
                      adhesion using
                      2,5-Dioxopyrrolidin-1-yl-6-acrylamidohexanoate as a
                      crosslinker to immobilize matrix proteins on the gels
                      surface. Finally we present an easy method to generate
                      polyacrylamide gels with a physiological Young's modulus of
                      55 kPa and defined surface ligand, facilitating the culture
                      of murine and human iPS-CMs, removing excess mechanical
                      stresses and reducing the risk of tissue culture artifacts
                      exerted by stiff substrates.},
      cin          = {ICS-7},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-7-20110106},
      pnm          = {453 - Physics of the Cell (POF2-453)},
      pid          = {G:(DE-HGF)POF2-453},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000339035000012},
      pubmed       = {pmid:24889032},
      doi          = {10.1016/j.biomaterials.2014.05.027},
      url          = {https://juser.fz-juelich.de/record/154975},
}