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@ARTICLE{Stadler:155323,
author = {Stadler, Andreas and Garvey, Christopher J. and Embs, Jan
Peter and Koza, Michael Marek and Unruh, Tobias and Artmann,
Gerhard and Zaccai, Giuseppe},
title = {{P}icosecond dynamics in haemoglobin from different
species: {A} quasielastic neutron scattering study},
journal = {Biochimica et biophysica acta / General subjects},
volume = {1840},
number = {10},
issn = {0304-4165},
address = {Amsterdam [u.a.]},
publisher = {Elsevier},
reportid = {FZJ-2014-04496},
pages = {2989 - 2999},
year = {2014},
abstract = {BackgroundDynamics in haemoglobin from platypus
(Ornithorhynchus anatinus), chicken (Gallus gallus
domesticus) and saltwater crocodile (Crocodylus porosus)
were measured to investigate response of conformational
motions on the picosecond time scale to naturally occurring
variations in the amino acid sequence of structurally
identical proteins.MethodsProtein dynamics was measured
using incoherent quasielastic neutron scattering. The
quasielastic broadening was interpreted first with a simple
single Lorentzian approach and then by using the
Kneller–Volino Brownian dynamics model.ResultsMean square
displacements of conformational motions, diffusion
coefficients of internal dynamics and residence times for
jump-diffusion between sites and corresponding effective
force constants (resilience) and activation energies were
determined from the data.ConclusionsModifications of the
physicochemical properties caused by mutations of the amino
acids were found to have a significant impact on protein
dynamics. Activation energies of local side chain dynamics
were found to be similar between the different proteins
being close to the energy, which is required for the rupture
of single hydrogen bond in a protein.General significanceThe
measured dynamic quantities showed significant and
systematic variations between the investigated species,
suggesting that they are the signature of an evolutionary
adaptation process stimulated by the different physiological
environments of the respective protein.},
cin = {ICS-1 / Neutronenstreuung ; JCNS-1},
ddc = {570},
cid = {I:(DE-Juel1)ICS-1-20110106 / I:(DE-Juel1)JCNS-1-20110106},
pnm = {451 - Soft Matter Composites (POF2-451) / 54G - JCNS
(POF2-54G24)},
pid = {G:(DE-HGF)POF2-451 / G:(DE-HGF)POF2-54G24},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000341675900001},
doi = {10.1016/j.bbagen.2014.06.007},
url = {https://juser.fz-juelich.de/record/155323},
}