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000155679 1001_ $$0P:(DE-Juel1)132018$$aSchröder, Gunnar$$b0$$eCorresponding Author
000155679 245__ $$aDeformable elastic network refinement for low-resolution macromolecular crystallography
000155679 260__ $$aCopenhagen$$bMunksgaard$$c2014
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000155679 520__ $$aCrystals of membrane proteins and protein complexes often diffract to low resolution owing to their intrinsic molecular flexibility, heterogeneity or the mosaic spread of micro-domains. At low resolution, the building and refinement of atomic models is a more challenging task. The deformable elastic network (DEN) refinement method developed previously has been instrumental in the determinion of several structures at low resolution. Here, DEN refinement is reviewed, recommendations for its optimal usage are provided and its limitations are discussed. Representative examples of the application of DEN refinement to challenging cases of refinement at low resolution are presented. These cases include soluble as well as membrane proteins determined at limiting resolutions ranging from 3 to 7 Å. Potential extensions of the DEN refinement technique and future perspectives for the interpretation of low-resolution crystal structures are also discussed.
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000155679 7001_ $$0P:(DE-HGF)0$$aLevitt, Michael$$b1
000155679 7001_ $$0P:(DE-HGF)0$$aBrunger, Axel T.$$b2
000155679 773__ $$0PERI:(DE-600)2020492-9$$a10.1107/S1399004714016496$$gVol. 70, no. 9, p. 2241 - 2255$$n9$$p2241 - 2255$$tActa crystallographica / D$$v70$$x1399-0047$$y2014
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