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@ARTICLE{Banachowicz:155846,
      author       = {Banachowicz, Ewa and Patkowski, Adam and Meier, G. and
                      Klamecka, Kamila and Gapiński, Jacek},
      title        = {{S}uccessful {FCS} {E}xperiment in {N}onstandard
                      {C}onditions},
      journal      = {Langmuir},
      volume       = {30},
      number       = {29},
      issn         = {1520-5827},
      address      = {Washington, DC},
      publisher    = {ACS Publ.},
      reportid     = {FZJ-2014-04804},
      pages        = {8945 - 8955},
      year         = {2014},
      abstract     = {Fluorescence correlation spectroscopy (FCS) is frequently
                      used to measure the self-diffusion coefficient of
                      fluorescently labeled probes in solutions, complex media,
                      and living cells. In a standard experiment water immersion
                      objectives and window thickness in the range of 0.13–0.19
                      mm are used. We show that successful FCS measurements can be
                      performed using samples of different refractive index placed
                      in cells having windows of different thickness, even much
                      thicker than nominally allowed. Different water, oil, and
                      silicon oil immersion as well as long working distance dry
                      objectives, equipped with the correction collar, were tested
                      and compared. We demonstrate that the requirements for FCS
                      experiments are less stringent than those for high
                      resolution confocal imaging and reliable relative FCS
                      measurements can be performed even beyond the compensation
                      range of the objectives. All these features open new
                      possibilities for construction of custom-made high
                      temperature and high pressure cells for FCS.},
      cin          = {ICS-3},
      ddc          = {670},
      cid          = {I:(DE-Juel1)ICS-3-20110106},
      pnm          = {451 - Soft Matter Composites (POF2-451)},
      pid          = {G:(DE-HGF)POF2-451},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000339686700037},
      doi          = {10.1021/la5015708},
      url          = {https://juser.fz-juelich.de/record/155846},
}