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000156269 0247_ $$2DOI$$a10.1371/journal.pone.0106882
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000156269 037__ $$aFZJ-2014-05060
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000156269 1001_ $$0P:(DE-Juel1)145441$$aFrenzel, Daniel$$b0$$ufzj
000156269 245__ $$aKinetic Titration Series with Biolayer Interferometry
000156269 260__ $$aLawrence, Kan.$$bPLoS$$c2014
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000156269 520__ $$aBiolayer interferometry is a method to analyze protein interactions in real-time. In this study, we illustrate the usefulness to quantitatively analyze high affinity protein ligand interactions employing a kinetic titration series for characterizing the interactions between two pairs of interaction patterns, in particular immunoglobulin G and protein G B1 as well as scFv IC16 and amyloid beta (1–42). Kinetic titration series are commonly used in surface plasmon resonance and involve sequential injections of analyte over a desired concentration range on a single ligand coated sensor chip without waiting for complete dissociation between the injections. We show that applying this method to biolayer interferometry is straightforward and i) circumvents problems in data evaluation caused by unavoidable sensor differences, ii) saves resources and iii) increases throughput if screening a multitude of different analyte/ligand combinations.
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000156269 7001_ $$0P:(DE-Juel1)132029$$aWillbold, Dieter$$b1$$eCorresponding Author$$ufzj
000156269 773__ $$0PERI:(DE-600)2267670-3$$n9$$pe106882$$tPLoS one$$v9$$x1932-6203$$y2014
000156269 8564_ $$uhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0106882
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