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@ARTICLE{Frenzel:156269,
      author       = {Frenzel, Daniel and Willbold, Dieter},
      title        = {{K}inetic {T}itration {S}eries with {B}iolayer
                      {I}nterferometry},
      journal      = {PLoS one},
      volume       = {9},
      number       = {9},
      issn         = {1932-6203},
      address      = {Lawrence, Kan.},
      publisher    = {PLoS},
      reportid     = {FZJ-2014-05060},
      pages        = {e106882},
      year         = {2014},
      abstract     = {Biolayer interferometry is a method to analyze protein
                      interactions in real-time. In this study, we illustrate the
                      usefulness to quantitatively analyze high affinity protein
                      ligand interactions employing a kinetic titration series for
                      characterizing the interactions between two pairs of
                      interaction patterns, in particular immunoglobulin G and
                      protein G B1 as well as scFv IC16 and amyloid beta (1–42).
                      Kinetic titration series are commonly used in surface
                      plasmon resonance and involve sequential injections of
                      analyte over a desired concentration range on a single
                      ligand coated sensor chip without waiting for complete
                      dissociation between the injections. We show that applying
                      this method to biolayer interferometry is straightforward
                      and i) circumvents problems in data evaluation caused by
                      unavoidable sensor differences, ii) saves resources and iii)
                      increases throughput if screening a multitude of different
                      analyte/ligand combinations.},
      cin          = {ICS-6},
      ddc          = {500},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {452 - Structural Biology (POF2-452)},
      pid          = {G:(DE-HGF)POF2-452},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000342123900015},
      pubmed       = {pmid:25229647},
      doi          = {10.1371/journal.pone.0106882},
      url          = {https://juser.fz-juelich.de/record/156269},
}