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@ARTICLE{Kirchenbuechler:156365,
author = {Kirchenbuechler, Inka and Guu, Donald and Kurniawan,
Nicholas A. and Koenderink, Gijsje H. and Lettinga, M.P.},
title = {{D}irect visualization of flow-induced conformational
transitions of single actin filaments in entangled
solutions},
journal = {Nature Communications},
volume = {5},
issn = {2041-1723},
address = {London},
publisher = {Nature Publishing Group},
reportid = {FZJ-2014-05117},
pages = {5060},
year = {2014},
abstract = {While semi-flexible polymers and fibres are an important
class of material due to their rich mechanical properties,
it remains unclear how these properties relate to the
microscopic conformation of the polymers. Actin filaments
constitute an ideal model polymer system due to their
micron-sized length and relatively high stiffness that allow
imaging at the single filament level. Here we study the
effect of entanglements on the conformational dynamics of
actin filaments in shear flow. We directly measure the full
three-dimensional conformation of single actin filaments,
using confocal microscopy in combination with a
counter-rotating cone-plate shear cell. We show that
initially entangled filaments form disentangled
orientationally ordered hairpins, confined in the
flow-vorticity plane. In addition, shear flow causes
stretching and shear alignment of the hairpin tails, while
the filament length distribution remains unchanged. These
observations explain the strain-softening and shear-thinning
behaviour of entangled F-actin solutions, which aids the
understanding of the flow behaviour of complex fluids
containing semi-flexible polymers.},
cin = {ICS-7 / ICS-3},
ddc = {500},
cid = {I:(DE-Juel1)ICS-7-20110106 / I:(DE-Juel1)ICS-3-20110106},
pnm = {453 - Physics of the Cell (POF2-453)},
pid = {G:(DE-HGF)POF2-453},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000343976900003},
pubmed = {pmid:25297898},
doi = {10.1038/ncomms6060},
url = {https://juser.fz-juelich.de/record/156365},
}