TY  - JOUR
AU  - Eekhoff, A.
AU  - Bonakdar, N.
AU  - Alonso, J.L.
AU  - Hoffmann, B.
AU  - Goldmann, W.H.
TI  - Glomerular podocytes: A study of mechanical properties and mechano-chemical signaling
JO  - Biochemical and biophysical research communications
VL  - 406
SN  - 0006-291X
CY  - Orlando, Fla.
PB  - Academic Press
M1  - PreJuSER-15795
SP  - 229 - 233
PY  - 2011
N1  - We thank Drs. Ben Fabry, Rudolf Merkel, Gerold Diez, James Smith, and Anna Klemm for helpful comments, Tim Feichtmeier for the cyclic stretch experiments on human umbilical cord fibroblasts, Andrea Zang for helping with OMTC, and Wolfgang Rubner for building a new cell stretcher. This work was supported by grants from Bayerisch-Franzosisches Hochschulzentrum, Deutscher Akademischer Austausch Dienst, Bavaria California Technology Center, and Deutsche Forschungsgemeinschaft.
AB  - Kidney glomeruli function as filters, allowing the passage of small solutes and waste products into the urinary tract, while retaining essential proteins and macromolecules in the blood stream. These structures are under constant mechanical stress due to fluid pressure, driving filtration across the barrier. We mechanically stimulated adherent wildtype podocytes using the methods of magnetic tweezer and twisting as well as cell stretching. Attaching collagen IV-coated or poly-l-lysine-coated magnetic beads to cell receptors allowed for the determination of cellular stiffness. Angiotensin II-treated podocytes showed slightly higher stiffness than untreated cells, the cell fluidity (i.e. internal dynamics) remained similar, and showed an increase with force. The bead detachment (a measure of the binding strength) was higher in angiotensin II-treated compared to untreated podocytes. Magnetic twisting confirmed that angiotensin II treatment of podocytes increases and CDTA treatment decreases cell stiffness. However, treatment with both angiotensin II and CDTA increased the cell stiffness only slightly compared to solely CDTA-treated cells. Exposing podocytes to cyclic, uniaxial stretch showed an earlier onset of ERK(1/2) phosphorylation compared to MEF (control) cells. These results indicate that angiotensin II might free intracellularly stored calcium and affects actomyosin contraction, and that mechanical stimulation influences cell signaling.
KW  - Angiotensin II: pharmacology
KW  - Angiotensin II: physiology
KW  - Animals
KW  - Cell Adhesion
KW  - Cytoskeleton: physiology
KW  - Kidney Glomerulus: cytology
KW  - Mechanical Processes
KW  - Mechanotransduction, Cellular
KW  - Mice
KW  - Mitogen-Activated Protein Kinase 1: metabolism
KW  - Mitogen-Activated Protein Kinase 3: metabolism
KW  - Podocytes: drug effects
KW  - Podocytes: physiology
KW  - Angiotensin II (NLM Chemicals)
KW  - Mitogen-Activated Protein Kinase 1 (NLM Chemicals)
KW  - Mitogen-Activated Protein Kinase 3 (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:21315064
UR  - <Go to ISI:>//WOS:000288616200013
DO  - DOI:10.1016/j.bbrc.2011.02.022
UR  - https://juser.fz-juelich.de/record/15795
ER  -